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ENZYME

ENZYME entry: EC 1.14.99.38

Accepted Name
cholesterol 25-monooxygenase
Alternative Name(s)
cholesterol 25-hydroxylase
Reaction catalysed
AH2 + cholesterol + O2 <=> 25-hydroxycholesterol + A + H2O
Comment(s)
  • Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P450.
  • Instead, it uses diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
  • The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues.
  • In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10.
Cross-references
BRENDA1.14.99.38
EC2PDB1.14.99.38
ExplorEnz1.14.99.38
PRIAM enzyme-specific profiles1.14.99.38
KEGG Ligand Database for Enzyme Nomenclature1.14.99.38
IUBMB Enzyme Nomenclature1.14.99.38
IntEnz1.14.99.38
MEDLINEFind literature relating to 1.14.99.38
MetaCyc1.14.99.38
Rhea expert-curated reactions1.14.99.38
UniProtKB/Swiss-Prot
Q5PRC0, CH25H_DANREO95992, CH25H_HUMANQ9Z0F5, CH25H_MOUSE
Q4G1G8, CH25H_PIGQ4QQV7, CH25H_RAT

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