ProtParam

TRYP_PIG (P00761)

Trypsin precursor (EC 3.4.21.4)
Sus scrofa (Pig)

The computation has been carried out on the complete sequence (231 amino acids).

Warning: All computation results shown below do not take into account
any annotated post-translational modification.
 References and documentation are available.

Number of amino acids: 231

Molecular weight: 24409.48

Theoretical pI: 7.00

Amino acid composition: 
Ala (A)  15	  6.5%
Arg (R)   4	  1.7%
Asn (N)  18	  7.8%
Asp (D)  10	  4.3%
Cys (C)  12	  5.2%
Gln (Q)  12	  5.2%
Glu (E)   5	  2.2%
Gly (G)  25	 10.8%
His (H)   4	  1.7%
Ile (I)  16	  6.9%
Leu (L)  16	  6.9%
Lys (K)  11	  4.8%
Met (M)   2	  0.9%
Phe (F)   5	  2.2%
Pro (P)  10	  4.3%
Ser (S)  27	 11.7%
Thr (T)  11	  4.8%
Trp (W)   4	  1.7%
Tyr (Y)   8	  3.5%
Val (V)  16	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	      1060
Hydrogen    H	      1662
Nitrogen    N	       296
Oxygen      O	       338
Sulfur      S	        14

Formula: C1060H1662N296O338S14
Total number of atoms: 3370

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    34670
Abs 0.1% (=1 g/l)   1.420, assuming all pairs of Cys residues form cystines


Ext. coefficient    33920
Abs 0.1% (=1 g/l)   1.390, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is F (Phe).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.71
This classifies the protein as stable.



Aliphatic index: 80.61

Grand average of hydropathicity (GRAVY): -0.111