ProtParam

User-provided sequence:

        10         20         30         40         50         60 
AFGADPPCVA KLQIATCYDP LTENAAFINV TPTSLIVSAF NPYCSAVQVG YQTSNFLQNA 

        70         80         90        100        110        120 
LGTIFSANDA TDAWSNYEIE GTVTNAFPVM ADGISQPNCT YINTTFILPS FNKTDNSAIT 

       130        140        150        160        170        180 
TLKPLFRTEQ GLYRAWADGA SETLVDAGAF TSQGLFTGNQ GVVEMVYTGC HPGHLNNCTV 

       190        200 
NTLDFRVATA VSVLVSFFTF INLFSM 

References and documentation are available.

Number of amino acids: 206

Molecular weight: 22139.88

Theoretical pI: 4.28

Amino acid composition: 
Ala (A)  22	 10.7%
Arg (R)   3	  1.5%
Asn (N)  17	  8.3%
Asp (D)   9	  4.4%
Cys (C)   6	  2.9%
Gln (Q)   8	  3.9%
Glu (E)   6	  2.9%
Gly (G)  13	  6.3%
His (H)   2	  1.0%
Ile (I)  10	  4.9%
Leu (L)  15	  7.3%
Lys (K)   3	  1.5%
Met (M)   3	  1.5%
Phe (F)  16	  7.8%
Pro (P)  10	  4.9%
Ser (S)  14	  6.8%
Thr (T)  24	 11.7%
Trp (W)   2	  1.0%
Tyr (Y)   7	  3.4%
Val (V)  16	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 6

Atomic composition:

Carbon      C	       994
Hydrogen    H	      1501
Nitrogen    N	       249
Oxygen      O	       307
Sulfur      S	         9

Formula: C994H1501N249O307S9
Total number of atoms: 3060

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21805
Abs 0.1% (=1 g/l)   0.985, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.968, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 20.89
This classifies the protein as stable.



Aliphatic index: 80.53

Grand average of hydropathicity (GRAVY): 0.207