ProtParam

User-provided sequence:

        10         20         30         40         50         60 
AHSNANSITI IQEAGKNKNN NAILTQLIVD LSSDVEEINM ETGERIIRRY KHIKANDEFK 

        70         80         90        100        110        120 
KVLSSLKNKR QLQLMTLEQA LELRELTNND KEVTNNTTAQ TEDEILKNLL VTQRYELKQK 

       130        140        150        160        170        180 
ILRKYRILRK RLKKTNQEQL RSLIEFDDNT QAEGNEDKSS EKLVELTKLS VQIKTKSISK 

       190        200        210        220        230        240 
LTKQLSKIEN DHQEHLDNYI SAMQKMSNVV NPINRSINTA IQSPYTIYDS PSVPSMIVMS 

       250        260        270        280        290        300 
NVDNKTEINN TNVHINLDTN SSNVHQNTGN QIMMQEYQQN MIENATLGEK NSITTLAGMK 

       310        320        330        340        350        360 
VLTNFFVVDG NKSAIHLATD GNKDQTNLLL EIRYQPSFDK IKSLVVDLYY VASPTTSKRK 

       370        380        390        400        410        420 
VIHHIVTQSQ SDIDFVTEHL LQFIRPFTRR TYSNHFLSSR MLPTQGQSNT SRISTGAEYL 

       430        440        450        460        470        480 
AEAIPLNGMS CAWHFATHLE PDIPADIYPA SHPPPHLLVQ PLSKNSKMEN SIFYDVLKSL 

       490        500        510        520        530        540 
YKNVKDNIDS TQPRSTVPYR PIMSIIPSTG GGPFREEFPN SDAFDGPSTP LSPSSSVSTA 

       550        560        570        580        590        600 
MSETQQTQIL SSQQQPVFGT NQNFVQQLQQ SPEVDSSLGD ELDIEKFQAS NNQQPQQQRS 

       610        620        630        640        650        660 
DHREEELIGL TPSQPGMTSR ISPLQTTSIS SNPIAPRPFG MDSQSRQLNV GLKRRSGRTS 

       670        680        690        700        710        720 
GVPRGRKRPA ATPNGLIKSS QFQNEEAKKN NRKTEMIAST SASRIAKKRD VKSAHHHKPA 

       730        740        750        760        770        780 
PPPSASGDTQ TFDFLPKLQD YVSFKEALQK AINLPVWTGQ YKGFGGQVKE HTEKLVEKEL 

       790        800        810        820        830 
IRTRKAKAFN AAKLIHTANV WDDKKRKMIS GTSHIFEYVD VGSYRASYIQ NSM 

References and documentation are available.

Number of amino acids: 833

Molecular weight: 93907.89

Theoretical pI: 9.27

Amino acid composition: 
Ala (A)  43	  5.2%
Arg (R)  40	  4.8%
Asn (N)  62	  7.4%
Asp (D)  39	  4.7%
Cys (C)   1	  0.1%
Gln (Q)  59	  7.1%
Glu (E)  49	  5.9%
Gly (G)  32	  3.8%
His (H)  22	  2.6%
Ile (I)  58	  7.0%
Leu (L)  66	  7.9%
Lys (K)  63	  7.6%
Met (M)  20	  2.4%
Phe (F)  25	  3.0%
Pro (P)  43	  5.2%
Ser (S)  86	 10.3%
Thr (T)  60	  7.2%
Trp (W)   3	  0.4%
Tyr (Y)  21	  2.5%
Val (V)  41	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 88
Total number of positively charged residues (Arg + Lys): 103

Atomic composition:

Carbon      C	      4099
Hydrogen    H	      6598
Nitrogen    N	      1184
Oxygen      O	      1298
Sulfur      S	        21

Formula: C4099H6598N1184O1298S21
Total number of atoms: 13200

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47790
Abs 0.1% (=1 g/l)   0.509, assuming all pairs of Cys residues form cystines


Ext. coefficient    47790
Abs 0.1% (=1 g/l)   0.509, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.52
This classifies the protein as unstable.



Aliphatic index: 77.49

Grand average of hydropathicity (GRAVY): -0.694