ProtParam

User-provided sequence:

        10         20         30         40         50         60 
AIPSTSAVIP RECMSSKYEH ANVQLQNMMN TTESSVNSKD SLSFEKVSND KDSMCNLKSH 

        70         80         90        100        110        120 
DRTTNYMGMV DSFTRFNLET NRKSVKTMRE QLYSSFRSMT LAMTDDTDKE KLPNFEQTML 

       130        140        150        160        170        180 
HAIEEEAFYD VSPLKPLGFM LSNIDTRETK FQVPRGYLRA CDMLPIIFRI MAELHHVGHH 

       190        200        210        220        230        240 
PLPRILLGNT RTTFNTIPKK TSRSTLSSVH SSNRRHGKPS FFTRSKPTTN PSENNLATSS 

       250        260        270        280        290        300 
SDGSGNNASS YISGSDVVDN FDTDSCFRVN GVLKIMQLGQ FFDNMNKSPE AANINYFFID 

       310        320        330        340        350        360 
QVYENDPIFF VCNKSSTSKD DRYFRIQYKG KADNDSSSKS NNTKSVKDIH CWVKVWGQGD 

       370        380        390        400        410        420 
LYVECWDKFN FPSNLIIHMD SMRLGYSSIV TGTLAKYISI FEFISIMCLR KFKELLGFDN 

       430        440        450        460        470        480 
CTISLQGNNF QLFEGVSSIE CQNINSPSDI IAVDAVQVSS NTIIIHSGLL SISEAEFVHG 

       490        500        510        520        530        540 
SSDKVNWVAE ADSSQFYIKN AHAQLLKFIP KTISFLEPPL ELFSRSSVPH SSLNISTASR 

       550        560 
SFKPLSSKIN VPKKLSDLIG M 

References and documentation are available.

Number of amino acids: 561

Molecular weight: 63206.51

Theoretical pI: 7.70

Amino acid composition: 
Ala (A)  22	  3.9%
Arg (R)  23	  4.1%
Asn (N)  42	  7.5%
Asp (D)  33	  5.9%
Cys (C)  10	  1.8%
Gln (Q)  16	  2.9%
Glu (E)  26	  4.6%
Gly (G)  22	  3.9%
His (H)  15	  2.7%
Ile (I)  38	  6.8%
Leu (L)  41	  7.3%
Lys (K)  37	  6.6%
Met (M)  19	  3.4%
Phe (F)  35	  6.2%
Pro (P)  23	  4.1%
Ser (S)  78	 13.9%
Thr (T)  33	  5.9%
Trp (W)   4	  0.7%
Tyr (Y)  14	  2.5%
Val (V)  30	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      2785
Hydrogen    H	      4353
Nitrogen    N	       759
Oxygen      O	       863
Sulfur      S	        29

Formula: C2785H4353N759O863S29
Total number of atoms: 8789

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43485
Abs 0.1% (=1 g/l)   0.688, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.678, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.33
This classifies the protein as unstable.



Aliphatic index: 74.35

Grand average of hydropathicity (GRAVY): -0.368