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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
ALGFGEHGEN IALLLSGRLT EYSEYPPLNL QNFCTHSSPL RESSGFDRHI SFKCVGNVGS 

        70         80         90        100        110        120 
LEKFGNLPVK STGTVFQLLK AREEADFSRV ARWFYSVEKC TATYNVYTTN NKWDDVDIDP 

       130        140        150        160        170        180 
LGSILLELEQ EDFITILDKS ILAYFGVLFN DMQEKVSTQL KYEVVKKVYD QKNAETVIID 

       190        200        210        220        230        240 
KGGEILNIVK HEGYDDTEVS FTEEIIDTID NELIWVLSKY YDPDLSEMDK LSVPRGLINK 

       250        260        270        280        290        300 
YVERSFHCDL FCQDRIAKGI IMGIFKFFSL HEPNIGSTRN PHFTTKDSPV PLFLAYDPNF 

       310        320        330        340        350        360 
MQRSLVQYWE RTVGGADVGS EGKFTIELKS NKIEDNTKFF LARYSDLFVQ ERRVTIPLKP 

       370        380        390        400        410        420 
REQNDSKLKA NFFYRKNDFD LVKPNKVLLA FPGSMLKPNS RIMQNLLKKH ADTFPFFLNE 

       430        440        450        460        470        480 
VRTKSFDLLK ADEYKVATNR NQSLDSRRCV VMLSEILPLL ITASTNIAQS KEFQSLCESL 

       490        500        510        520        530        540 
SSWVPGGNMK DYLSQLDKVN REEEKRKHTY LFDVTTLIKL LKAQDSSPLT FKQLLESNIE 

       550        560        570        580        590        600 
MGTTITGVER SLGDLASKLK SMISIALSVL HKTFISMCGQ LKSLNYIINL TQQFVRTTCS 

       610        620        630        640        650        660 
DLMIISLIKM LDDEDFSPLH FKKSLNEKGN SSTSLVALTK TCVQLIRTLL DMLVSEDTIL 

       670        680        690        700        710        720 
KRDLLSFLHW LPWRLKKDPL AEKRSKNNAK NVILNDHEAI LFYKLRSDAD ILSQLIEITQ 

       730        740        750        760        770        780 
NAVTLPTCDS PLQRVTTTKA HGMARSSIIN YVKELSHQDI IGESLIFLFT NMLDNRNQKS 

       790        800        810        820        830        840 
SCLRYFLEHY IERQIYPKSI FVSKMLSAIG ARDILPAFYI RAPKKKEPDA NNRDNSNGHD 

       850        860        870        880        890        900 
LGNEVEEDNN SVDDDARIVD ASSGIRGFMR QEEAETDLIG ERIDEPIAEL FDSDIERSHV 

       910        920        930        940        950        960 
NHDSNLRAEA RRERVHQAFV DARIDDPLAN MFEPDIDTGG IDVESGQITV YVPEHDDHTV 

       970        980        990       1000       1010       1020 
NSEQIDDQEV SIADSSNHSE VKAENFLLNK NKLKEQERKN ELDKQLALSV KYLRNIVTPI 

      1030       1040       1050       1060       1070       1080 
ICNAYTKSDY FMDFIDKWRS VTSKLIIGDL VPEDFFSGSV LVESFHLHHS RPRSGSGAVT 

      1090       1100       1110       1120       1130       1140 
VQDSIDNEVQ ELGTPGLPNI LNSQNRRGRN LLSLSPASMA SHFHTRNISR HHNRHRMEDH 

      1150       1160       1170       1180       1190       1200 
VRFLPENENS FIHEIGRFVG RDDEESEGQS DDTLEVGEAI LWRRRSDGMR TSNIPEDESS 

      1210       1220       1230       1240       1250       1260 
ESDEDSISLS SLGSDWDSED VDYDSLDVDY GSSLEDDYVE IVDMSADDEN ESHSSHSINA 

      1270       1280       1290       1300       1310       1320 
DYDAMEEDDG SPQDINIILG DADDVEYDVD TGDATSSQME SSLENEESDS RADDADEEGM 

      1330       1340       1350       1360       1370       1380 
DDDEDSFVVE FGNDSDVFAM ADDDGILSTD DDDDEEIDEV DYMGLASNKF MDPIEEKDSE 

      1390       1400       1410       1420       1430       1440 
DVEDEVEDHD EIKFHESFNN RTSSITNLAE VAVNFIKKSF PYNLDISAMA ETIVSPLGLD 

      1450       1460       1470       1480       1490       1500 
IVLKCLQYQD AEVKNSDLLQ RLYPAQVYVM STIIRFWMDL KLHNSELLKS SSTANRIAKI 

      1510       1520       1530       1540       1550       1560 
IAEIAFKRIF NMHPDALKVD TKTTRDDQVT ALFGIIVSRA LMGIVERRKA EHKDHDTGVP 

      1570       1580       1590       1600       1610       1620 
KDLLRYLFFN IATTRPRPRE AYTNRRSFTL FEFKCQNYSS VLEVLLKLLF IMYACVPNRS 

      1630       1640       1650       1660       1670       1680 
PDLQAKKNEP LDSSNAPESL WDKESAAMLQ SLLLHMIGTR EYIDSKETSS AQTNKDKEGL 

      1690       1700       1710       1720       1730       1740 
FRKLSYDVLA GQDDFEHFRA TECLVDIFQS PSRMVVHAKE ELITTLERVA DDGGGLPRAT 

      1750       1760       1770       1780       1790       1800 
FSRNIEARIL ADVNETTEFC RRTLLLFSSE LFNIKDLKQF SGIVKLLRSL IGSRAINNAY 

      1810       1820       1830       1840       1850       1860 
LEDRSYFILI RSTALASYFN SIDSVRILID FLKQKMPDPI RFVKAMSLLP YRLLVDKSLE 

      1870       1880       1890       1900       1910       1920 
ELHPLESKAL IIEYVYLAKS FWPTNVHEPK LSKELYEIFR QMLHSAKQYI YKENLFIGFL 

      1930       1940       1950       1960       1970       1980 
HILSSLTAPD DLDTELIRDL IRGAFVETPE DYKTFIQLLT KAFANVLKPY FPLVTFIRSP 

      1990       2000       2010       2020       2030       2040 
LGDNFFTDRL EDLEKSSCGK YYQAQMPQPY YISKDEGILK KYKEWQEPSF LGISNDAPLD 

      2050       2060       2070       2080       2090       2100 
DGTDTLYNAA VDGPIGVQEF LSIKKIPPFI LDWHLEYLEK TYINVNTFIN KLMSLLQKFV 

      2110       2120       2130       2140       2150       2160 
SYPVNRSQTK FLSDSKDLDL IMALAMVKIP VTLAKMINYD DDISVYYKGI SQVNEMTETQ 

      2170       2180       2190       2200       2210       2220 
MSKNYFTLAN ILCSLTLIGN SEDIYNINDL SSLDKIKTMR TFLDRIIHLY LLYGGKQYFI 

      2230       2240       2250       2260       2270       2280 
YAPITQLIET SNPSAKPITF VFTNFNLLVL IYPLHGELET AKLMEIVNEV VNEFIHVETT 

      2290       2300       2310       2320       2330       2340 
LDKHELDMNR SHSLRLFCRF LIATNSQLTY LITRMQLTNR TKVSTIKGKQ DGKKPDDIYI 

      2350       2360       2370       2380       2390       2400 
QLPPGDGSVD VIPLPMTETI VSDPMHQRIY MEELACKQFL LKLNKILSFG RKDFYSYIQM 

      2410       2420       2430       2440       2450       2460 
IRKCSLTTLD IYVNTVINLI AALRCLKSHC SRVSNEGDRE LFFSRDNPSN LFDNLNELQD 

      2470       2480       2490       2500       2510       2520 
NLVKMVDMFA YDEYKEDLYQ LVETVNSIAV STFYEYPPNE MFIIALFDRA ELHQPVQSFS 

      2530       2540       2550       2560       2570       2580 
GNGLSMGFLV STFTDLMTGK DLLEWYAPEH EEKDTLIEEN KRHFFYKQDK PSTFLFEREN 

      2590       2600       2610       2620       2630       2640 
RFTSPMKRIV NCLAEVINAK LDPYHRALED IDTGYETSRD GGTLIRAHDA DLLFDFLNNI 

      2650       2660       2670       2680       2690       2700 
LGKEKVQKLG EPNLCIASVV DPLLSLLEAS PGILKPFNDI VYPILGAEVL VPYITPESNI 

      2710       2720       2730       2740       2750       2760 
VKQVVDLTYT ILTLGFVLKN ELIKKLSVLI PSDILNRIRD GSDTSLLKLV LKLLSRIYAL 

      2770       2780       2790       2800       2810       2820 
ERFSISYYVV SYKPPQWTEP HALTFDIENA VSTILMTFGD NEIFETTTES NDIFTELLHT 

      2830       2840       2850       2860       2870       2880 
ASALTRKYPC NRISVIELLT PILGAAFLSE HLPKVDALNS ILYFFRVNYE EDIEDTAERL 

      2890       2900       2910       2920       2930       2940 
TKAIYRLIQF LLGHSINGGL NRIIDSCWVH KLSICELTFL ADTRLEKPIK TESLSILDTL 

      2950       2960       2970       2980       2990       3000 
SNFAYPDLEF LKSSVQPPSF ITNVFAIANL KTEIIINRLD INEGSDDSFA KAVSAKISFD 

      3010       3020       3030       3040       3050       3060 
FWSEAPLLAM GKDFIQQLSH ECLEQTTMTV KKISTQTENI NNKQQSSKKF DNSTYHTFRL 

      3070       3080       3090       3100       3110       3120 
KRWKQPVKKK DFYLDVLSFH EGDEDMVSSS LSLALKLTKK RLNHDGFINH AVIKERAIVF 

      3130       3140       3150       3160       3170       3180 
REGLIALIRM VALKITFNPC NLLYMLVDGS SYVFRNETNL LLRRTFKLMK LCYDVDHAEM 

      3190       3200       3210       3220       3230       3240 
EVLKVECEKK PDQKYKYKEA VKLLLGDMRD LIPIWVYLDD RSRDWEQIAE IRELFTPTDC 

      3250       3260 
DILYDVLPKY GAALKEKRAK ECRTFLVM


References and documentation are available. 

Number of amino acids: 3268

Molecular weight: 374184.78

Theoretical pI: 5.06


Amino acid composition: 
Ala (A) 161	  4.9%
Arg (R) 157	  4.8%
Asn (N) 171	  5.2%
Asp (D) 265	  8.1%
Cys (C)  36	  1.1%
Gln (Q)  94	  2.9%
Glu (E) 241	  7.4%
Gly (G) 120	  3.7%
His (H)  72	  2.2%
Ile (I) 237	  7.3%
Leu (L) 377	 11.5%
Lys (K) 213	  6.5%
Met (M)  73	  2.2%
Phe (F) 168	  5.1%
Pro (P) 120	  3.7%
Ser (S) 272	  8.3%
Thr (T) 176	  5.4%
Trp (W)  22	  0.7%
Tyr (Y) 113	  3.5%
Val (V) 180	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%



Total number of negatively charged residues (Asp + Glu): 506
Total number of positively charged residues (Arg + Lys): 370

Atomic composition:

Carbon      C	     16742
Hydrogen    H	     26293
Nitrogen    N	      4383
Oxygen      O	      5107
Sulfur      S	       109

Formula: C16742H26293N4383O5107S109
Total number of atoms: 52634

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   291620
Abs 0.1% (=1 g/l)   0.779, assuming all pairs of Cys residues form cystines


Ext. coefficient   289370
Abs 0.1% (=1 g/l)   0.773, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.19
This classifies the protein as unstable.



Aliphatic index: 94.17

Grand average of hydropathicity (GRAVY): -0.297