ProtParam

User-provided sequence:

        10         20         30         40         50         60 
DEDPIYLHPQ AGTATPEEED PIILAEDENE QQQASPAPQE RDRARQAVID DLKEKVYPLL 

        70         80         90        100        110        120 
TVWFLVFNKI VATIVRDNVP DPIIYRLVVD LVNHEAIQSC LTQFWRRYKP LTNIDKKQSE 

       130        140        150        160        170        180 
KWIMLLLENL SDQPYLTNMN RTTEYLEFFE KFLEDLADKP IEGSLVKIFL LNLQGVDPTN 

       190        200        210        220        230        240 
TDYEIDYKSY VSLRQEHLNP IMLLDIASQT DKCKKYFNLT LSNSNIFEKI QSFKVEDIEN 

       250        260        270        280        290        300 
LHLYDRWLNW VEQTNWVTRF IFLLSLLIIY KWPLFSGFEL IIFFPVLSNY GILYFTRILV 

       310        320        330        340        350        360 
LLLPFSNRLP RVVRRNWASE DNEVTNNDNA SSTNDAARVH EGSPHLLTSD RPREIKFNLA 

       370        380        390        400        410        420 
SELQEIASPS LLINDGNIRY DNSSIPIKIE GYDGKIVSLY SSQPNLESVP IFVVEDGNII 

       430        440        450        460        470        480 
CKRYLEDTPV IDNGSETVYP IGREEGRIAA PSRGQTPAFS VPSQSRTDPP MFQFINPNNF 

       490        500        510        520        530        540 
VENWFGPGSE TYEQNIQVHV KVEHPEKIYE SIKSSNGAFN NEHLLQGKYI LKIDNRRLNL 

       550        560        570        580        590        600 
SHTYVSCIFD KVQKVTTDPK VNMQTISLTA DSIFELVFRE EDNAIHSNQE HNMKFLFTFF 

       610        620        630        640        650        660 
SNHAPAFDIG ILDVLNLDKY YDSDSGYNLF MPTQKGKIIF GQLDSVSHDE KIGCLNGASK 

       670        680        690        700        710        720 
EFDKIRELAL KELRALTTGI PLNPEMSNFI RQSSLTNIEF QINMSNFEGE MDYELDFASG 

       730        740        750        760        770        780 
SPSTRKELQL RIFHNSDPHS SSTIDKLLCT ASLERGEYSL FYTNISNLPT HPIESNTCQE 

       790        800        810        820        830 
EPQYIARLQK YIQYHIYQLL RGVTSLPHAN VLLNTRILDI ESSWITFKCP TQALYESM 

References and documentation are available.

Number of amino acids: 838

Molecular weight: 96653.32

Theoretical pI: 4.99

Amino acid composition: 
Ala (A)  33	  3.9%
Arg (R)  37	  4.4%
Asn (N)  61	  7.3%
Asp (D)  50	  6.0%
Cys (C)   8	  1.0%
Gln (Q)  37	  4.4%
Glu (E)  65	  7.8%
Gly (G)  29	  3.5%
His (H)  19	  2.3%
Ile (I)  70	  8.4%
Leu (L)  89	 10.6%
Lys (K)  41	  4.9%
Met (M)  11	  1.3%
Phe (F)  44	  5.3%
Pro (P)  46	  5.5%
Ser (S)  68	  8.1%
Thr (T)  44	  5.3%
Trp (W)  10	  1.2%
Tyr (Y)  34	  4.1%
Val (V)  42	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      4358
Hydrogen    H	      6728
Nitrogen    N	      1136
Oxygen      O	      1313
Sulfur      S	        19

Formula: C4358H6728N1136O1313S19
Total number of atoms: 13554

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   106160
Abs 0.1% (=1 g/l)   1.098, assuming all pairs of Cys residues form cystines


Ext. coefficient   105660
Abs 0.1% (=1 g/l)   1.093, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is D (Asp).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.14
This classifies the protein as unstable.



Aliphatic index: 92.47

Grand average of hydropathicity (GRAVY): -0.362