ProtParam

User-provided sequence:

        10         20         30         40         50         60 
DLDDDIEMSD ENDDDGLTDA GITAEHNPLV ELCEEAEEPV NEDRSKGKKL VVNVTEKDKI 

        70         80         90        100        110        120 
ENKLIRLALK NLIENQILRN LPRAGMDDSY GYKALFDKAE QTLNLKYHKD NQEFREEIEK 

       130        140        150        160        170        180 
LRIDVIKHIA KRSLKNFIVI SSIRNLFEPR FHQRVAGMVL NKTSEQIKSG QQSNIFEAGL 

       190        200        210        220        230        240 
NSTMIVICNS CDITKGQGST IRGDDLMQLM VTLVDPHAKE VEDFLLVSYP KYQLQNTLFG 

       250        260        270        280        290        300 
GEDYGVYGAT TGLLKSVAYK ESLESCDVRI MMDEDNFLFG AVKKALETKG SGSLGLFLFS 

       310        320        330        340        350        360 
APQRPNALGS RSLRVANSVA KIADMQGVVE SSLDREMHIL KENESESLKK VPIGTLRAAT 

       370        380        390        400        410        420 
ESITDSDVVN QIMSNAGARR EEEAVQDELK EIQKKIDPIA FYRLDAATAT DYRREADLAK 

       430        440        450        460        470        480 
NELEDLKKKA QTLEEHGHKE ENYRQRLPEL EEQLSAEKQR ALKLRDKTTS DADEDRELAK 

       490        500        510        520        530        540 
IEVQILQLQR EKSDLEEPKS DRAVAVGACS IDVLDLASDP LRRYPLYRKA LQAATVLASD 

       550        560        570        580        590        600 
LIRVGHHIEY KPQLGRLIAV TQRVSPEAVE IKQFRREFAG DKEVISRYEN NTTAGIVKLQ 

       610        620        630        640        650        660 
GRSLAPKLIN AADDKGNGML MHIEDIFLVI LTKSEEIEKL VGKFREEFDG KYKAGATLAA 

       670        680        690        700        710        720 
LDLSFLKAGQ LITPVDDDII RQAVGEIIAT KGIGPEGILC PNSKIRRALV RITSRIEEER 

       730        740        750        760        770        780 
GIVPDLKGQR AQETMDIAYK SLYELPTNTD AGRSDIRTNG RLELAQQKIA EIDVQAEKFI 

       790        800        810        820        830        840 
QQISSDNFLA FLIHDQAIFS DKQQKQIKAA DQLVKGLAYS PTIEAPAPQQ QPIRVLNRNV 

       850        860        870        880        890        900 
VKKFLDYDYR GKEILNQLYP VSGDEPTEIF AALIHIPQLQ PHQHDSALKQ ALTLITLARE 


TFQTQDNM 

References and documentation are available.

Number of amino acids: 908

Molecular weight: 102034.97

Theoretical pI: 5.31

Amino acid composition: 
Ala (A)  77	  8.5%
Arg (R)  54	  5.9%
Asn (N)  37	  4.1%
Asp (D)  68	  7.5%
Cys (C)   6	  0.7%
Gln (Q)  52	  5.7%
Glu (E)  82	  9.0%
Gly (G)  48	  5.3%
His (H)  15	  1.7%
Ile (I)  69	  7.6%
Leu (L)  97	 10.7%
Lys (K)  70	  7.7%
Met (M)  15	  1.7%
Phe (F)  26	  2.9%
Pro (P)  30	  3.3%
Ser (S)  51	  5.6%
Thr (T)  40	  4.4%
Trp (W)   0	  0.0%
Tyr (Y)  22	  2.4%
Val (V)  49	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 150
Total number of positively charged residues (Arg + Lys): 124

Atomic composition:

Carbon      C	      4480
Hydrogen    H	      7285
Nitrogen    N	      1259
Oxygen      O	      1411
Sulfur      S	        21

Formula: C4480H7285N1259O1411S21
Total number of atoms: 14456

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33155
Abs 0.1% (=1 g/l)   0.325, assuming all pairs of Cys residues form cystines


Ext. coefficient    32780
Abs 0.1% (=1 g/l)   0.321, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is D (Asp).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.99
This classifies the protein as unstable.



Aliphatic index: 95.43

Grand average of hydropathicity (GRAVY): -0.468