ProtParam
User-provided sequence:
10 20 30 40 50 60
DNRIHVINDV KILHNDIDRG NLLDNSRFKE RGEAITTVLG SQLHRKAVRE ETAEYDIDIK
70 80 90 100 110 120
YVLLAGTDAL TKYIKKWNEQ DYLLYLGELI VIRTFKSICY QDPTPDKLAH NFGPVFIDPI
130 140 150 160 170 180
TQSFTKSLKE FVSSTSYFKH HSSVKCLSTK ALIKCLQLFN NSDFTSPSGR RKHATQPDKF
190 200 210 220 230 240
LDLCRRSLHF GDMPVIQAIN ISDQALKNVN VPNDVAAIRI ANATGGRAVV ATCEENNSTL
250 260 270 280 290 300
ASYKVPQFNV DEVHDKFLGQ NEIMEAVKNP QLTKLSDVLN VMPKLRDNVE IVNPHESLFT
310 320 330 340 350
HYKENIIQCL EEAITSKGSG PSGVLIVCVR YNDEIRNDLL QLVDDALKHT DVM
References and
documentation are available.
Number of amino acids: 353
Molecular weight: 39885.46
Theoretical pI: 6.33
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1768
Hydrogen H 2820
Nitrogen N 490
Oxygen O 537
Sulfur S 11
Formula: C
1768H
2820N
490O
537S
11
Total number of atoms: 5626
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20775
Abs 0.1% (=1 g/l) 0.521, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.511, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is D (Asp).
The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.39
This classifies the protein as stable.
Aliphatic index: 96.06
Grand average of hydropathicity (GRAVY): -0.313