ProtParam

User-provided sequence:

        10         20         30         40         50         60 
DVEEMETDAP VEEVPAATSA EPATETEEDE DINLGLSILR NIRSAFSTPE DLSFGSTLLA 

        70         80         90        100        110        120 
TEYLLKTLDK VTKDQAGGED VRKKLEKIIP SKPSIEFTKK SSMYSSMSSD RLAQAKMIRE 

       130        140        150        160        170        180 
MNASWGFQGT RIAAPADLLK YSVVVKEVQD GLIEKLAKTL PEYEKIEKER EAKEEDTEEL 

       190        200        210        220        230        240 
EFDKTIDVLT KGEFEKLQTF AYEDIPDTLF LVEFNKAKLA DLFPSKEVAK LSEGTIYYIN 

       250        260        270        280        290        300 
KQHEPMRTVY DTLSTLEDVS KTSNYRLLKA LAARNQTDEH VGLKINKSFA SYFKEFQESD 

       310        320        330        340        350        360 
EAIENFAEIL KKVINKRIVK MIKNQQLMER SLNLPLDESD VVGKVFSLWE PILDEAEDTI 

       370        380        390        400        410        420 
FVRRVYLKIN NKKKKSEFLD FPARKPIFLI ARFELQGEVS FHKVYLPDEW DNSISKYFAN 

       430        440        450        460        470        480 
YEEQTIDSPN RTWLPKTKNL EEIEQVEEKV KKTKPKKEEE EDVEELKPKK DDEDKKEEDK 

       490        500        510        520        530        540 
KEEDKKEEEP IPVEKEVEKT VVLQIPYAVF ESHRKIVEKI RKEELYELQD DKLFLRLITG 

       550        560        570        580        590        600 
RGIRENVEDL TVTFSGGANS EWIYQEDDNS KSIVQVRDAV LFLSYFGVGF QGIMSVDAGA 

       610        620        630        640        650        660 
SLAEMFAKTG SKAITGLNNI LEAKTMGIGS DRIELVKQEP KPTIRIFLDP ETELQKPDSL 

       670        680        690        700 
SKYRIKDLAD SANSILERLF IEKNSYVTNI ILSMLQTIEA QFEFTESAM 

References and documentation are available.

Number of amino acids: 709

Molecular weight: 81406.40

Theoretical pI: 4.84

Amino acid composition: 
Ala (A)  43	  6.1%
Arg (R)  27	  3.8%
Asn (N)  26	  3.7%
Asp (D)  47	  6.6%
Cys (C)   0	  0.0%
Gln (Q)  22	  3.1%
Glu (E)  95	 13.4%
Gly (G)  24	  3.4%
His (H)   4	  0.6%
Ile (I)  49	  6.9%
Leu (L)  66	  9.3%
Lys (K)  77	 10.9%
Met (M)  13	  1.8%
Phe (F)  33	  4.7%
Pro (P)  27	  3.8%
Ser (S)  49	  6.9%
Thr (T)  40	  5.6%
Trp (W)   5	  0.7%
Tyr (Y)  20	  2.8%
Val (V)  42	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 142
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      3641
Hydrogen    H	      5798
Nitrogen    N	       928
Oxygen      O	      1151
Sulfur      S	        13

Formula: C3641H5798N928O1151S13
Total number of atoms: 11531

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57300
Abs 0.1% (=1 g/l)   0.704

Estimated half-life:

The N-terminal of the sequence considered is D (Asp).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.78
This classifies the protein as unstable.



Aliphatic index: 86.50

Grand average of hydropathicity (GRAVY): -0.575