ProtParam

User-provided sequence:

        10         20         30         40         50         60 
DYEQLCDGPV IGRDENLYRK DDVSVHISGK QTNCKEVLCW GDTHTALIKV KEGLSIRIED 

        70         80         90        100        110        120 
TLRPEYDQIV TYTKGLQVSA EGNYKSNPTL PLPKSRSLHQ LHQYMKSQRR KKRKKLPKSP 

       130        140        150        160        170        180 
TPIILEHKET SKNCDHHDNF NTELTQQATT GYIVSHPRAT ILDINNNRYS TIQSDPTTNG 

       190        200        210        220        230        240 
LNNVSDNADL PTNNYELIPS REDPMIDSEN FYGKSIRSLM KMQKLQPAGT PTKLSTKSLG 

       250        260        270        280        290        300 
YDKFYTSGRL FWRSLPSEYS WKESTKLATK KSSVTKQSPL DNYSPMSSEI HVINPPKTYL 

       310        320        330        340        350        360 
FADVVKEEEM ENVKPDLSIN KGSWAYPNKV TKKPTLIHQP IIPKSIKYQI KSSMNDGSTW 

       370        380        390        400        410        420 
QTDNYKEIDR NSYSNQDECK SQWFLRSFWN TRRCYEEERP ITASMPPNSI SVSNRKLYFY 

       430        440        450        460        470        480 
CLLILLGFCF IGIPLGISLG ITSASSSKPQ AAVNSFLLNS PTSRISTAIT TVTTVSTSSS 

       490        500        510 
AQSSIYNSAL TTSMAAVTTL VTTTTQMITA VM 

References and documentation are available.

Number of amino acids: 512

Molecular weight: 57779.42

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  19	  3.7%
Arg (R)  21	  4.1%
Asn (N)  32	  6.2%
Asp (D)  24	  4.7%
Cys (C)   8	  1.6%
Gln (Q)  21	  4.1%
Glu (E)  25	  4.9%
Gly (G)  19	  3.7%
His (H)  10	  2.0%
Ile (I)  35	  6.8%
Leu (L)  41	  8.0%
Lys (K)  41	  8.0%
Met (M)  11	  2.1%
Phe (F)  11	  2.1%
Pro (P)  31	  6.1%
Ser (S)  62	 12.1%
Thr (T)  49	  9.6%
Trp (W)   7	  1.4%
Tyr (Y)  23	  4.5%
Val (V)  22	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 62

Atomic composition:

Carbon      C	      2546
Hydrogen    H	      4042
Nitrogen    N	       696
Oxygen      O	       798
Sulfur      S	        19

Formula: C2546H4042N696O798S19
Total number of atoms: 8101

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73270
Abs 0.1% (=1 g/l)   1.268, assuming all pairs of Cys residues form cystines


Ext. coefficient    72770
Abs 0.1% (=1 g/l)   1.259, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is D (Asp).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.77
This classifies the protein as unstable.



Aliphatic index: 74.06

Grand average of hydropathicity (GRAVY): -0.604