ProtParam

User-provided sequence:

        10         20         30         40         50         60 
EEVDIQDNPE KQEQGEAWQT IDDVYSLITE ISDPNNSELP LFQVMGFDDV LNAICQNLRL 

        70         80         90        100        110        120 
FKPNSAQNMG ETEMLLMADP NLFRKLKKKN IDGKILDLKS LVNIHPLELL IMASMASLAG 

       130        140        150        160        170        180 
SFFKSSDIVF PAELLYTACL NFNLQQTLHR VINPLVPIHT YLEIQGPCDF ILYEDNFDGI 

       190        200        210        220        230        240 
EEDLWDLNKL LYEFCYILAG NPGLDMEEMV DDLSILDRID ITFEYKTAEA APDLNVIHAR 

       250        260        270 
RGVTQMHSII SNCFTSKGAG APGLVMVGVR SM 

References and documentation are available.

Number of amino acids: 272

Molecular weight: 30653.13

Theoretical pI: 4.37

Amino acid composition: 
Ala (A)  16	  5.9%
Arg (R)   7	  2.6%
Asn (N)  18	  6.6%
Asp (D)  22	  8.1%
Cys (C)   5	  1.8%
Gln (Q)  11	  4.0%
Glu (E)  20	  7.4%
Gly (G)  14	  5.1%
His (H)   5	  1.8%
Ile (I)  22	  8.1%
Leu (L)  35	 12.9%
Lys (K)  12	  4.4%
Met (M)  11	  4.0%
Phe (F)  13	  4.8%
Pro (P)  13	  4.8%
Ser (S)  15	  5.5%
Thr (T)  10	  3.7%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.6%
Val (V)  14	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	      1369
Hydrogen    H	      2146
Nitrogen    N	       346
Oxygen      O	       418
Sulfur      S	        16

Formula: C1369H2146N346O418S16
Total number of atoms: 4295

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21680
Abs 0.1% (=1 g/l)   0.707, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.699, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.05
This classifies the protein as stable.



Aliphatic index: 102.54

Grand average of hydropathicity (GRAVY): -0.036