ProtParam
User-provided sequence:
10 20 30 40 50 60
ELLNKCLKYC GKQSKAWLRK WIPKNKLNQE VDDRDVLYKP HHLLEQVFSA LIFNFYFRFI
70 80 90 100 110 120
LSVLTILITM TYEYSETASQ KSIDIGRETL KGTSIPNSQL ANNGDINATD ELTFWQTTFW
130 140 150 160 170 180
LTFFCTSITD ITYLVCIQSF VLLNPKHILY LGQSFVILTF VSWLYAIWQM FDLPHGTFLA
190 200 210 220
LIGYAGSCKN LISIGLVIEV ALYLPMFGLF SKPLTLLSSR M
References and
documentation are available.
Number of amino acids: 221
Molecular weight: 25483.89
Theoretical pI: 8.28
Total number of negatively charged residues (Asp + Glu): 16
Total number of positively charged residues (Arg + Lys): 18
Atomic composition:
Carbon C 1193
Hydrogen H 1830
Nitrogen N 282
Oxygen O 317
Sulfur S 9
Formula: C
1193H
1830N
282O
317S
9
Total number of atoms: 3631
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 48150
Abs 0.1% (=1 g/l) 1.889, assuming all pairs of Cys residues form cystines
Ext. coefficient 47900
Abs 0.1% (=1 g/l) 1.880, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is E (Glu).
The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 39.28
This classifies the protein as stable.
Aliphatic index: 110.72
Grand average of hydropathicity (GRAVY): 0.318