ProtParam
User-provided sequence:
10 20 30 40 50 60
FDDWKDEDQE NKKDNNDIKQ YETHTTNGAT SSDEANGNFN KTFNSFAQFG YSSTEQFKQG
70 80 90 100 110 120
FQAAARKTEE SLEGSQWQQV HPKIVTENVD EFSKTVASSF LGWGRSLTGL PDAQFNELSL
130 140 150 160 170 180
TNSSGASREQ PPKAPTSGFG QYKGGQSPPL HDPRSQNKKG LEAFYAENKE KQFNAGNSSN
190 200 210 220 230 240
APTASRRNEL PTGERSQAVG PTASAATQST ASLKSADFDL HEREEFVRDE CLCTLKEKYD
250 260 270 280 290 300
EAVPNDYKVK QPLSLDINHS KFWETLPENG GKEMRLLEEP KFQDMTISRV FSIHVGLGRH
310 320 330 340 350
IGACELCIFA GFKPTAWQPN PAGCDMCKKN AGIKQLQLLR RRTDPDVKWD SM
References and
documentation are available.
Number of amino acids: 352
Molecular weight: 39296.31
Theoretical pI: 5.65
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1713
Hydrogen H 2635
Nitrogen N 489
Oxygen O 556
Sulfur S 10
Formula: C
1713H
2635N
489O
556S
10
Total number of atoms: 5403
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42315
Abs 0.1% (=1 g/l) 1.077, assuming all pairs of Cys residues form cystines
Ext. coefficient 41940
Abs 0.1% (=1 g/l) 1.067, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is F (Phe).
The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.95
This classifies the protein as unstable.
Aliphatic index: 51.08
Grand average of hydropathicity (GRAVY): -0.924