ProtParam

User-provided sequence:

        10         20         30         40         50         60 
FGMVKLIGYG VTVIAAYSLL SAPVGRTIFT GVTLYRDGVE DIMSIATVNP FGSTPLGMAS 

        70         80         90        100        110        120 
SCLLAAIVIL LRSHDGSPLN SGIESMLPVI IMAAVTHSVF TAMVLIVLGF ILVIIFIPEH 

       130        140        150        160        170        180 
EVQAKILQAM TSLLGSSSVA KGLTTGGMAL VVITWMFNNF DDSTLLGTGF FVVIPIISII 

       190        200        210        220        230        240 
GMEGFIGSIQ NSLCWLVITI ICVLTVFWQK LTFPDRSPHL QLIKVNPEPP FTIILLVWIA 

       250        260        270        280        290        300 
MVCIFCVPLA IMFWEAWSPS PDMIGISFIN QPSAIPSSMG GINSALAIGL ILAKAYSCNR 

       310        320        330        340        350        360 
PLTRLLPQVI SYCLVPAAVN SVWMSVFLAV FMNTLLIFHP NTGASALIHT SLVKAINYKS 

       370        380        390        400        410        420 
LAAALTFGGL LLMIVSSWMT SLIFQSSDRP SMPQSTIPDK IVPFVVILLP IMLSTVFLPI 

       430        440        450        460        470        480 
TETAWLLSAY ILIAFCNKQL SDQTFPTLNF TLLAIFCSTI LLFKLLSTQG MIFQLWQKPP 

       490        500        510        520        530        540 
LHMLERFTLS DTSPAIIGAG GSSMAMSETI NANPRLQSSS LKSFKKNDTK ASQYKRELNM 

       550        560        570        580        590        600 
MDKWVTNREW VVHDRLHSSL EREADQFDLV EQRNGDEDEE NGSSNEDNNI RDADLNFTNS 

       610        620        630        640        650        660 
PRRTGKNLSA YTLITETIHH QIRNITAPNF VHSHFKIYNL YNQKLNTNLS KDFKKCIKSF 

       670        680        690        700        710        720 
GTQNLEIFDK LESLQTYISI LRKKLSLRHQ TFPSLYVPNS NSLSVSASAL NQMSNGNNTT 

       730        740        750        760        770        780 
NSVQTHIVPA SDISMDTLSP NSDTSSVSSF RRQRQQSKSK RNRAVSQLQH QQQQNFLQTA 

       790        800        810        820        830        840 
KNTNELEMVD DKLENYNAIL GTEQSIYFKD IKKLEQNLAA VFKSIYFQDD PSADALLPLN 

       850        860        870        880        890 
EDNADHPEVV HHKNNSYLKD KQLSYILKKL QSYALYKTSW EPVSNFQLSH SFKM 

References and documentation are available.

Number of amino acids: 894

Molecular weight: 99490.36

Theoretical pI: 8.19

Amino acid composition: 
Ala (A)  56	  6.3%
Arg (R)  25	  2.8%
Asn (N)  55	  6.2%
Asp (D)  35	  3.9%
Cys (C)  10	  1.1%
Gln (Q)  41	  4.6%
Glu (E)  30	  3.4%
Gly (G)  37	  4.1%
His (H)  20	  2.2%
Ile (I)  76	  8.5%
Leu (L) 105	 11.7%
Lys (K)  43	  4.8%
Met (M)  30	  3.4%
Phe (F)  47	  5.3%
Pro (P)  42	  4.7%
Ser (S)  97	 10.9%
Thr (T)  57	  6.4%
Trp (W)  13	  1.5%
Tyr (Y)  20	  2.2%
Val (V)  55	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 68

Atomic composition:

Carbon      C	      4501
Hydrogen    H	      7109
Nitrogen    N	      1161
Oxygen      O	      1295
Sulfur      S	        40

Formula: C4501H7109N1161O1295S40
Total number of atoms: 14106

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   101925
Abs 0.1% (=1 g/l)   1.024, assuming all pairs of Cys residues form cystines


Ext. coefficient   101300
Abs 0.1% (=1 g/l)   1.018, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is F (Phe).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.71
This classifies the protein as unstable.



Aliphatic index: 103.06

Grand average of hydropathicity (GRAVY): 0.158