ProtParam

User-provided sequence:

        10         20         30         40         50         60 
GEWNIFNFDN ENTDLLNLDY DPGQDEVMGI NGFKLNQSHV NQNENTSTQQ PGLTTGVNSS 

        70         80         90        100        110        120 
PQILTNDNDG SNTSASTEDA VNSNENPTRN NSAYAQNPFL LSSSNKLADF DYGQNNSSPK 

       130        140        150        160        170        180 
KEEPTSLTNR NGKSSRSDNG VQAELINGGN PLEQSVTNNQ STIIKSNKLT INLNTTSFGH 

       190        200        210        220        230        240 
PPKIVVNGPV KSSRQKQADG SRPTAISLPS SASVKSNVAR KPGNKGAIKG SEPDFCSETV 

       250        260        270        280        290        300 
SSLPSSVAPK NFSGLNIGPF PLSNATKTPP ASHINPAITN SFTRLMNNED KTADANLIVS 

       310        320        330        340        350        360 
PTSKRRSVRT NPPSRKKGTV VSNGGLKNTS EVPFASTSAK KTNKRVKTPQ KSALASPSAG 

       370        380        390        400        410        420 
DLSPSSKSNS YIDEEGFIIA QNRFNTAIDS RPTKNVRSGV MAPESTTSGP TTINPSCANT 

       430        440        450        460        470        480 
SGASVTATNN KRRRKAVLGA KVKGKAKSTA SRSRTSKSTP VNNPKDTSHM TKLSKPQNVP 

       490        500        510        520        530        540 
SKNNTTNNTT NNNCPNGVPN ETTPQMPYNY VPWGTVNQSR NQTPGVNTFD GSPPATPAVA 

       550        560        570        580        590        600 
TAGTTPNANN YPPISFNNVI PNGVMPIPIP NMNVNRMQVA PAMPNGLMMA ALHMPDIDEN 

       610        620        630        640        650        660 
SYEGRREADH QLRAAHVALS RYIQEQRQEQ LVLQYYQQAF ESGGRSSSTN FIDWFIQWEY 

       670        680        690        700        710        720 
LFGQPTDVVK SFTNQNGNNE KSKPGDVPNQ GKDRDLHADQ AFAAATNKLS SKTLFDFIYE 

       730        740        750        760        770        780 
NLTNKCNMKE KLDSNEATPQ NIWQQQQQQQ QQQQQRQQES NTAMAINSQL DQKYQSAMYP 

       790 
QETPPISDPY SSNVKYSM 

References and documentation are available.

Number of amino acids: 798

Molecular weight: 86648.36

Theoretical pI: 9.35

Amino acid composition: 
Ala (A)  55	  6.9%
Arg (R)  30	  3.8%
Asn (N)  98	 12.3%
Asp (D)  33	  4.1%
Cys (C)   4	  0.5%
Gln (Q)  52	  6.5%
Glu (E)  31	  3.9%
Gly (G)  44	  5.5%
His (H)   8	  1.0%
Ile (I)  31	  3.9%
Leu (L)  38	  4.8%
Lys (K)  50	  6.3%
Met (M)  16	  2.0%
Phe (F)  23	  2.9%
Pro (P)  64	  8.0%
Ser (S)  92	 11.5%
Thr (T)  66	  8.3%
Trp (W)   5	  0.6%
Tyr (Y)  17	  2.1%
Val (V)  41	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      3706
Hydrogen    H	      5883
Nitrogen    N	      1109
Oxygen      O	      1252
Sulfur      S	        20

Formula: C3706H5883N1109O1252S20
Total number of atoms: 11970

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53080
Abs 0.1% (=1 g/l)   0.613, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   0.610, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.36
This classifies the protein as unstable.



Aliphatic index: 55.51

Grand average of hydropathicity (GRAVY): -0.891