ProtParam

User-provided sequence:

        10         20         30         40         50         60 
HGGWTSNGGY NSKNGQNNWA SRNGDDQNGW ASAGGDRTSK NNNNGGWTSA GGYASRDGTG 

        70         80         90        100        110        120 
GWTSMAGNSE GGSAWTSAGG WSSKNGWASA GGWTSTAGTG QGGWASAGGG AGGWASAGGN 

       130        140        150        160        170        180 
GQGGWTSAGG WSSVGGGGSG GHANVAPTKG GDFSSWGPTM GGSLGSSTAA AGAAIGGTAM 

       190        200        210        220        230        240 
NRPASVYSPG MRAQPVRGRR NVLEDYTIGE GGERNYYTLK HKDITIHKNK SHLEVTAKDG 

       250        260        270        280        290        300 
NVDKVIGLQG KYGASRIRVT KGLAVERGGR SQIPQQFTKS SPPGMKSIIE PNMKSLDMKN 

       310        320        330        340        350        360 
SLMNDKSAVA EVNSPNVVFV GANEVIKKSV VFIQQTQIYL VQGERRSGVK EVITDGIKIE 

       370        380        390        400        410        420 
NGNADVSSVR ARATNIKSLI SGKTITSVKG TEDIVKFIDH GAQIICAVNK ASLEVIDHLA 

       430        440        450        460        470        480 
YESSTATTDI SKSLNNATIT VERSTQTSMF TVQGQEVMLV LGADGQHEGN IVTVHDGPEF 

       490        500        510        520        530        540 
IKRLTSIPFE LPTTFGNLKI TAIDKTTRTV IGKSGRQEGN LVEIRDGPQF TVKAAQAKKI 

       550        560        570        580        590        600 
SQSVSTLDVA GEKSGFRALE EVTPQINKTE VHQIRFSKYL YGDIYDENKY TFHRDDRKYL 

       610        620        630        640        650        660 
NAQDLRLAMT PNFLQPPARH AFTPRRSKRQ QTTPDIEDFK GYDLRPVIKL MVELNNESIQ 

       670        680        690        700        710        720 
DVMALDGKYI GRKIRVYSGE ELAVDDSKNP KLLLPLEQVP ILLKQSIYID PVGNCFKEIV 

       730        740        750        760        770        780 
SQKPAEIYIR GTYNDRQFIS LIKLKKKGMA RDLNFKKKLL KRILEKEKGP RCRVGWITAT 

       790        800        810        820        830        840 
DVSPLLFRQP VYGDQAAARY QKSSSRGYRE RLEKALRQAD EESTKNLDQD LQRHLRDDRR 

       850        860        870        880        890        900 
AGPASAEDDE DDGHTIFGER VLESDEEDED EDEDDSVEAE IDLFRNREQR RRKTPAEDDD 

       910        920        930        940        950        960 
DEDDDDDDDD EDDDEDKNDG EDKTDGDSNK TDEERPRKRE DSGEEKKVIE NAESSDTNLT 

       970        980        990       1000       1010       1020 
AQADDTAPGN SSSVQETAAT EANDEPQQPE SKAEKEEGDL PPINSTSEAR ETSETTTVNG 

      1030       1040       1050       1060 
SDVTDKDSTN EDKTDTSQPV VVPDAFQQDH DQMSVNTDSN DSM 

References and documentation are available.

Number of amino acids: 1063

Molecular weight: 115649.83

Theoretical pI: 5.09

Amino acid composition: 
Ala (A)  78	  7.3%
Arg (R)  60	  5.6%
Asn (N)  58	  5.5%
Asp (D)  92	  8.7%
Cys (C)   3	  0.3%
Gln (Q)  48	  4.5%
Glu (E)  76	  7.1%
Gly (G) 109	 10.3%
His (H)  15	  1.4%
Ile (I)  55	  5.2%
Leu (L)  54	  5.1%
Lys (K)  73	  6.9%
Met (M)  16	  1.5%
Phe (F)  23	  2.2%
Pro (P)  39	  3.7%
Ser (S)  91	  8.6%
Thr (T)  74	  7.0%
Trp (W)  15	  1.4%
Tyr (Y)  23	  2.2%
Val (V)  61	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 168
Total number of positively charged residues (Arg + Lys): 133

Atomic composition:

Carbon      C	      4951
Hydrogen    H	      7861
Nitrogen    N	      1467
Oxygen      O	      1694
Sulfur      S	        19

Formula: C4951H7861N1467O1694S19
Total number of atoms: 15992

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   116895
Abs 0.1% (=1 g/l)   1.011, assuming all pairs of Cys residues form cystines


Ext. coefficient   116770
Abs 0.1% (=1 g/l)   1.010, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is H (His).

The estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro).
                            10 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.67
This classifies the protein as stable.



Aliphatic index: 63.97

Grand average of hydropathicity (GRAVY): -0.832