ProtParam

User-provided sequence:

        10         20         30         40         50         60 
HSLFWRGKLK RRKLIKSGQK SKARALFGFT RKRKLTSPQY TNGRSKWRRQ GILGFGLPTF 

        70         80         90        100 
SPTKLPSSNL LMGTQPRLVS LASFSSISSF MRRSQPQCLR AFLPM 

References and documentation are available.

Number of amino acids: 105

Molecular weight: 12030.25

Theoretical pI: 12.53

Amino acid composition: 
Ala (A)   4	  3.8%
Arg (R)  13	 12.4%
Asn (N)   2	  1.9%
Asp (D)   0	  0.0%
Cys (C)   1	  1.0%
Gln (Q)   6	  5.7%
Glu (E)   0	  0.0%
Gly (G)   8	  7.6%
His (H)   1	  1.0%
Ile (I)   3	  2.9%
Leu (L)  14	 13.3%
Lys (K)  10	  9.5%
Met (M)   3	  2.9%
Phe (F)   8	  7.6%
Pro (P)   7	  6.7%
Ser (S)  15	 14.3%
Thr (T)   6	  5.7%
Trp (W)   2	  1.9%
Tyr (Y)   1	  1.0%
Val (V)   1	  1.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 0
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       542
Hydrogen    H	       884
Nitrogen    N	       166
Oxygen      O	       136
Sulfur      S	         4

Formula: C542H884N166O136S4
Total number of atoms: 1732

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12490
Abs 0.1% (=1 g/l)   1.038, assuming all pairs of Cys residues form cystines


Ext. coefficient    12490
Abs 0.1% (=1 g/l)   1.038, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is H (His).

The estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro).
                            10 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 63.68
This classifies the protein as unstable.



Aliphatic index: 69.71

Grand average of hydropathicity (GRAVY): -0.511