ProtParam

User-provided sequence:

        10         20         30         40         50         60 
IDHVSIKLKA QIINSVQSDT KIEEGSGDFL KTQLKFNDTD IRPAIKSRLE KYTTDGKLML 

        70         80         90        100        110        120 
AFIDDKYYFK IKTTKLGSTS QAPAQKSGSL SLDSLKKNLK LDEGTLTNDQ NTSAFNSTQS 

       130        140        150        160        170        180 
ATAFTATDNE QLTKINNQNE DREFERDFGN NLVVFLSHVM ESRSIYDPLN VLDAVYINLD 

       190        200        210        220        230        240 
EKRKKTISNT VYPVPGPIYP MIRKSWQGGA DRLKGAEAPF ADLLQVQLDY FDQYYRKLQR 

       250        260        270        280        290        300 
TKGNSLECCV LFWYKEDELG FSEVSAKVLL EGDVLKASKN QPKTISQQQQ QEVSGLSINS 

       310        320        330        340        350        360 
AKYRAINSKW EQVTPLNVSK IDEIVDNGTA YGTAIDIISV FGVPVLGPGG LRGIPKAIFW 

       370        380        390        400        410        420 
ECNHHACIFL NEGVYTTLED AKEAKFDYLV IAYLSGMNLS DNTVKRSSSN SGNVSSPKTR 

       430        440        450        460        470        480 
DFVEFYTKPV VGEKGTSPNS AKYWDKEDGS VYFFEGEMFS LEKSTQAQYS YKAKIVKEPS 

       490        500        510        520        530        540 
TIDKSNHRNK PSSDRQSKVP TSSSSLYRVP VTKIHKIVSG NDHSPTSIDA STIRGKSGNS 

       550 
DRKSLKFNKL M 

References and documentation are available.

Number of amino acids: 551

Molecular weight: 61605.37

Theoretical pI: 8.71

Amino acid composition: 
Ala (A)  29	  5.3%
Arg (R)  19	  3.4%
Asn (N)  33	  6.0%
Asp (D)  37	  6.7%
Cys (C)   4	  0.7%
Gln (Q)  24	  4.4%
Glu (E)  30	  5.4%
Gly (G)  32	  5.8%
His (H)   7	  1.3%
Ile (I)  33	  6.0%
Leu (L)  43	  7.8%
Lys (K)  54	  9.8%
Met (M)   6	  1.1%
Phe (F)  23	  4.2%
Pro (P)  21	  3.8%
Ser (S)  60	 10.9%
Thr (T)  35	  6.4%
Trp (W)   5	  0.9%
Tyr (Y)  22	  4.0%
Val (V)  34	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 73

Atomic composition:

Carbon      C	      2734
Hydrogen    H	      4316
Nitrogen    N	       738
Oxygen      O	       860
Sulfur      S	        10

Formula: C2734H4316N738O860S10
Total number of atoms: 8658

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60530
Abs 0.1% (=1 g/l)   0.983, assuming all pairs of Cys residues form cystines


Ext. coefficient    60280
Abs 0.1% (=1 g/l)   0.978, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is I (Ile).

The estimated half-life is: 20 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.97
This classifies the protein as stable.



Aliphatic index: 76.95

Grand average of hydropathicity (GRAVY): -0.566