ProtParam

User-provided sequence:

        10         20         30         40         50         60 
IHHFIATIFR AFFPPNLNHG GNEKRTSSSS TPSLMGTSST SSTSKSISLA TTSTQTKQGK 

        70         80         90        100        110        120 
SSSSSTASHS TSAFYSISSN AATSFMNGGS VISEYTSWTS SYGPAKLASP VASEIIEIYE 

       130        140        150        160        170        180 
GDDSFNAQAM SIEMNDLDYV VYTNALFNDG LIITPDSQPA IGLICINSRS DTVLQFDSLW 

       190        200        210        220        230        240 
NSLYFGGFDF IISSEEEMEK ICDMIYYGYA SSYTAGVQDA LMKVLETPMY SITTGSDLLV 

       250        260        270        280        290        300 
PIKTTTLTTL NDEKDGKSTG LGQLTVQFQI PDKYGRHQLT NIIPITYLDG AYKGHDVAGF 

       310        320        330        340        350        360 
LITGHKSDSD NAFLSYATSK IVGSNKLVMP FNNYTYSKQV SSLSVGSYTS ELGPLGIGLV 

       370        380        390        400        410        420 
GVTSNTENAV AFSLGTINLD NLSLQDHGWT GSAFTTDGYA ISFETNNSNF TSSKSTNFTG 

       430        440        450        460        470        480 
YTACDILQST SATSDFTATA ESTVITTDYS IETFVSEVVS ALADKNVQKF SSGTTDKKKT 

       490        500        510        520        530        540 
SCYPNDSGTV WLDSSGTDVL VTVKQPPTGI DLEVSYFSNQ NTLEVLEYDD DRTMLRTRGK 

       550        560        570        580        590 
TDDLANEFSS GYKKQFNMKV YVENSPLSPF DTVGADVISS CVLLADVVFT LVSLKM 

References and documentation are available.

Number of amino acids: 596

Molecular weight: 64269.23

Theoretical pI: 4.56

Amino acid composition: 
Ala (A)  34	  5.7%
Arg (R)   7	  1.2%
Asn (N)  32	  5.4%
Asp (D)  39	  6.5%
Cys (C)   5	  0.8%
Gln (Q)  17	  2.9%
Glu (E)  23	  3.9%
Gly (G)  41	  6.9%
His (H)   8	  1.3%
Ile (I)  35	  5.9%
Leu (L)  46	  7.7%
Lys (K)  28	  4.7%
Met (M)  12	  2.0%
Phe (F)  29	  4.9%
Pro (P)  18	  3.0%
Ser (S)  88	 14.8%
Thr (T)  67	 11.2%
Trp (W)   4	  0.7%
Tyr (Y)  27	  4.5%
Val (V)  36	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      2837
Hydrogen    H	      4382
Nitrogen    N	       714
Oxygen      O	       952
Sulfur      S	        17

Formula: C2837H4382N714O952S17
Total number of atoms: 8902

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62480
Abs 0.1% (=1 g/l)   0.972, assuming all pairs of Cys residues form cystines


Ext. coefficient    62230
Abs 0.1% (=1 g/l)   0.968, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is I (Ile).

The estimated half-life is: 20 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.95
This classifies the protein as stable.



Aliphatic index: 76.22

Grand average of hydropathicity (GRAVY): -0.159