ProtParam

User-provided sequence:

        10         20         30         40         50         60 
IKKQLKMYFL HYDDLDYVIK DLKIYDPLSE NISLLKQFSQ NQYNKTFFTM SFERPQFVNL 

        70         80         90        100        110        120 
IHLLVDLNDQ KGQSIDFVPI NSEFSAYSWG KEPTVHLTYY YKEALIMNSS YGCPTFAFAD 

       130        140        150        160        170        180 
HHFSLDSDQA SNVYIEDLRT NKTMQYGLNH GKDENPEVLA TTSAEPGCVF KSACEPDLET 

       190        200        210        220        230        240 
MLVEFTEDDD EIYEKGKPTS RDTETVYLNW HNSKDNRGVS YSKGNDFFKN LYDVEDAWNQ 

       250        260        270        280        290        300 
HIAAQKCPFL FCRRSYFVKF PKYKGGQTTR FAWSLEQEVI QFLKELCFLT TTTGCTKMTL 

       310        320        330        340        350        360 
KHDFVFLSSE SLLFADLEKT KKMSLVECKV LKLIEIWRDM GINRLTKETT ISKKHPFFWI 

       370        380        390 
ELLKEPGEFA DTSDLTASLE HDIYNHNTLE KITVTM 

References and documentation are available.

Number of amino acids: 396

Molecular weight: 46232.42

Theoretical pI: 5.38

Amino acid composition: 
Ala (A)  15	  3.8%
Arg (R)   9	  2.3%
Asn (N)  20	  5.1%
Asp (D)  28	  7.1%
Cys (C)   8	  2.0%
Gln (Q)  14	  3.5%
Glu (E)  30	  7.6%
Gly (G)  14	  3.5%
His (H)  12	  3.0%
Ile (I)  19	  4.8%
Leu (L)  40	 10.1%
Lys (K)  35	  8.8%
Met (M)   9	  2.3%
Phe (F)  28	  7.1%
Pro (P)  13	  3.3%
Ser (S)  28	  7.1%
Thr (T)  31	  7.8%
Trp (W)   6	  1.5%
Tyr (Y)  19	  4.8%
Val (V)  18	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      2096
Hydrogen    H	      3176
Nitrogen    N	       522
Oxygen      O	       625
Sulfur      S	        17

Formula: C2096H3176N522O625S17
Total number of atoms: 6436

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61810
Abs 0.1% (=1 g/l)   1.337, assuming all pairs of Cys residues form cystines


Ext. coefficient    61310
Abs 0.1% (=1 g/l)   1.326, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is I (Ile).

The estimated half-life is: 20 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.42
This classifies the protein as stable.



Aliphatic index: 75.08

Grand average of hydropathicity (GRAVY): -0.461