ProtParam

User-provided sequence:

        10         20         30         40         50         60 
LGPPGQLGPP TPKNIDNGMN NSFGPPGSLG PPPAISSQVT TMGPPPPLSF KPQESEPPSQ 

        70         80         90        100        110        120 
LPPPAIGPVG SSFSSSTLGP PPTPTVNQAQ RSIHSAPPSP SPAYRSSAPF KLPPETSNND 

       130        140        150        160        170        180 
YRSRKPAQIP HTNSNYHVDN SQIPASNNVT ANRDFRSMRQ SSSNCDRSFH GTQGCIKCVI 

       190        200        210        220        230        240 
GQINPIKRNP CDYRKHDKLG CIPCPRNDER LTGNLEALER LQGRKLDNQG EPSTVAKIVI 

       250        260        270        280        290        300 
NQCVKIGKQI KDESDAIILC HLPDEFNMAG PPLDSANKGE KVSGRGRIAI KCNSDEQLKR 

       310        320        330        340        350        360 
LTRGRPGLLL GVFNVDPYQD VPIYYKDQFK TPRKYDDPPV FYPITKLAIE VLKIREDELK 

       370        380        390        400        410        420 
KRYRQERTNT RKGQADYVPP PSPSRNKRSP PVFDNTRLKI TIEQIRFMVQ YSYIQERTLA 

       430        440        450        460        470 
GSIKSPLSNF RHVDSEVTNK RWLSLNVNPP VKAKKEEMSS GKRNEFYRSN IRRFSM 

References and documentation are available.

Number of amino acids: 476

Molecular weight: 53034.17

Theoretical pI: 9.72

Amino acid composition: 
Ala (A)  19	  4.0%
Arg (R)  36	  7.6%
Asn (N)  33	  6.9%
Asp (D)  23	  4.8%
Cys (C)   9	  1.9%
Gln (Q)  23	  4.8%
Glu (E)  21	  4.4%
Gly (G)  29	  6.1%
His (H)   7	  1.5%
Ile (I)  29	  6.1%
Leu (L)  29	  6.1%
Lys (K)  33	  6.9%
Met (M)   7	  1.5%
Phe (F)  15	  3.2%
Pro (P)  59	 12.4%
Ser (S)  46	  9.7%
Thr (T)  21	  4.4%
Trp (W)   1	  0.2%
Tyr (Y)  14	  2.9%
Val (V)  22	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2324
Hydrogen    H	      3710
Nitrogen    N	       688
Oxygen      O	       702
Sulfur      S	        16

Formula: C2324H3710N688O702S16
Total number of atoms: 7440

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26860
Abs 0.1% (=1 g/l)   0.506, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.497, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is L (Leu).

The estimated half-life is: 5.5 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.78
This classifies the protein as unstable.



Aliphatic index: 64.92

Grand average of hydropathicity (GRAVY): -0.829