ProtParam

User-provided sequence:

        10         20         30         40         50         60 
LPGNRLFAIL DIGSSDSSDN DNQAEIIPDP TTGPITPKVL YNESASADSY RLNKDEEISN 

        70         80         90        100        110        120 
EDDNTVINSS FFSSKVMSNT SVDQHKDHSK NQNLAFKLGP LSFHDNNYRI KLLRNLEDLD 

       130        140        150        160        170        180 
IGGLMPIHHI IRWFLPVEVE EATINNLMAQ LELSNSPLSR DSSNTKTEDS ETRKNDNSDK 

       190        200        210        220        230        240 
ASYYNEWYEF DDCNDYFSFH LKLEFLQKFF REVFWLIRKL PNTSYKWCKR KKWKGRYYWG 

       250        260        270        280        290        300 
RKGWNSHCFK LIEKEGLEGK LIYKEILYKS ETKAWSYNWP KEKFLYVIVD VLCSLPRVCT 

       310        320        330        340        350        360 
DIYTVWMTPG HKIEHNYQLA DAIIPFLWIG IYSLPYIFIA RLNKQIQARR KKMQKYTQAQ 

       370        380        390        400        410        420 
FHQQVNTFSS NECRDANNNV REQSFQQSPT FAENSLTPIN DAPNTNNNDV NNKINNSNNN 

       430        440        450        460        470        480 
NNNNNNNNNN NNNNNNNNNN NNNNNNNSNN NDNDNDNNNN NDDDDNNSNN NIIGGKEKIN 

       490        500        510        520        530        540 
STIDSNDNKN DYMALPSELS NQCSFTQHGL MSSKRTDLGE NEVSFTVKHE KQKSVLESPN 

       550        560        570        580        590        600 
LTIDNECDFP NNRELDPQES MAYKSMDLPK ASNYDLLRVG GSASVNEDSG TNTKRFLTPI 

       610        620        630        640        650        660 
ENSSEGFNFT GNSSGSDDTE ENPDIFSITF FNKLNRLLHL LPLKFYSRFY WKAKGWHGLG 

       670        680        690        700        710        720 
FLKKKEKEEE LVNHNFDGIQ AKIRKSESTI FIYISLYVAF IFIIIFYRPG WSLVIKYWYP 

       730        740        750        760        770        780 
KPPLYCWASW PKYGGQRPSD PFNYNNNDLI ITTDSDDNLK NYNIFALSAL IAPVLATIPW 

       790        800        810        820        830        840 
IYSRKKYLGG EMNGSRKNRW KWNPKFILIA FHIAFIMIAM DAGEIAFATF WGLTNFFAPT 

       850        860        870        880        890        900 
AYVSNNILII MPYIMLIFAK LFDCIILFAI LDHRFVKRRK DMKSLFFMGL CGAILSVASA 

       910        920        930        940        950        960 
TIAIIRLQLL QPATFTSTMG PLGLLQNVTT TNLQRLSDVR KELLSSGKLA NLNPPFGETI 


M 

References and documentation are available.

Number of amino acids: 961

Molecular weight: 110709.46

Theoretical pI: 6.68

Amino acid composition: 
Ala (A)  45	  4.7%
Arg (R)  36	  3.7%
Asn (N) 125	 13.0%
Asp (D)  58	  6.0%
Cys (C)  11	  1.1%
Gln (Q)  26	  2.7%
Glu (E)  49	  5.1%
Gly (G)  41	  4.3%
His (H)  17	  1.8%
Ile (I)  72	  7.5%
Leu (L)  86	  8.9%
Lys (K)  68	  7.1%
Met (M)  18	  1.9%
Phe (F)  55	  5.7%
Pro (P)  42	  4.4%
Ser (S)  80	  8.3%
Thr (T)  45	  4.7%
Trp (W)  21	  2.2%
Tyr (Y)  37	  3.9%
Val (V)  29	  3.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 104

Atomic composition:

Carbon      C	      4955
Hydrogen    H	      7571
Nitrogen    N	      1343
Oxygen      O	      1489
Sulfur      S	        29

Formula: C4955H7571N1343O1489S29
Total number of atoms: 15387

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   171255
Abs 0.1% (=1 g/l)   1.547, assuming all pairs of Cys residues form cystines


Ext. coefficient   170630
Abs 0.1% (=1 g/l)   1.541, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is L (Leu).

The estimated half-life is: 5.5 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.22
This classifies the protein as unstable.



Aliphatic index: 77.55

Grand average of hydropathicity (GRAVY): -0.584