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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
NPLERIYLVN NCRIFIEGLT GHSVGAVFEE AENLQLNFYN DTSVLTGRYE TSNFKLKVGV

70 80
RHNVLGKLFP KPNVPQMASI DSSESM


References and documentation are available.

Number of amino acids: 86 Molecular weight: 9659.98 Theoretical pI: 6.10
Amino acid composition: 
Ala (A) 3 3.5% Arg (R) 4 4.7% Asn (N) 9 10.5% Asp (D) 2 2.3% Cys (C) 1 1.2% Gln (Q) 2 2.3% Glu (E) 7 8.1% Gly (G) 6 7.0% His (H) 2 2.3% Ile (I) 4 4.7% Leu (L) 9 10.5% Lys (K) 4 4.7% Met (M) 2 2.3% Phe (F) 5 5.8% Pro (P) 4 4.7% Ser (S) 7 8.1% Thr (T) 4 4.7% Trp (W) 0 0.0% Tyr (Y) 3 3.5% Val (V) 8 9.3% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 9 Total number of positively charged residues (Arg + Lys): 8 Atomic composition: Carbon C 430 Hydrogen H 675 Nitrogen N 117 Oxygen O 130 Sulfur S 3 Formula: C430H675N117O130S3 Total number of atoms: 1355 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.463, assuming all pairs of Cys residues form cystines Ext. coefficient 4470 Abs 0.1% (=1 g/l) 0.463, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is N (Asn). The estimated half-life is: 1.4 hours (mammalian reticulocytes, in vitro). 3 min (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 28.31 This classifies the protein as stable. Aliphatic index: 89.42 Grand average of hydropathicity (GRAVY): -0.228