ProtParam

User-provided sequence:

        10         20         30         40         50         60 
NTSPKPENRK ENKEEEKRIK ETEYDNLYKD FADNREPFPR KGLLDIMDER TLVEKKLLVQ 

        70         80         90        100        110        120 
AIKEVDEAKE KLLKTCRDHC EQVIRYVESD IIDGTEDSFP KTLDSDDRKQ YNVWGIKDSM 

       130        140        150        160        170        180 
GLETVMATAM STVKKFDDSA GSTVSPFHLE ESVRGGLSMT MRDKLQQETL LFIDGPLYQA 

       190        200        210        220        230        240 
YGLAGQGRPI ISVKLLPDAY KLYWGCVAHG AEHYAVVKKE EPSLLKSKRE VGGIVREIAQ 

       250        260        270        280        290        300 
EFHNLKVADE DSRAAILAAE NCVNAIDAGS FGPTLAALRN KLDFIEGALK LHHLHVAFIA 

       310        320        330        340        350        360 
KRGELEPKDI NIHRDFRGPR LLAKDLIDPR NTGALVVVHD APTFGDMEVL MQNLTNERED 

       370        380        390        400        410        420 
NAGSFNGKQR AKGIADIEDI FVISPANERA TKFLDRVRAA GVGVFMEVFE SGSVFYFPVG 

       430        440        450        460        470        480 
AEGATAKALL TKGTGPPGSL IAGRPIKAGM KEYRSPEKLF SVFEMIEEKA EDCGAVDKFK 

       490        500        510        520        530        540 
IKVDTETNFK KAKSRSLGFI GGRSGGAAQA SRRTLWIIGA IMLVTPLIQF MAKAWNGEQV 

       550        560        570        580        590        600 
YLVPIRFDKD IDLEDQAKQL KHEFSDISGI TFYYFNRGYN DAQNKGNDNL MVKVMSKNVV 

       610        620        630        640        650        660 
ILKAVYGKAL LKERFDQWTI ESQEELSNLL DLLFSLLFFT LGIQFLNVNK SVPNSPDNLP 

       670        680        690        700        710        720 
PKPPPPPPPP PINRSNNGEF YGQEKTRNSK AKSPGNEEEK EKREEQIRNS EQKLVADELR 

       730        740        750        760        770        780 
RISEDIKRKQ EKWNAPITNN KTQEIYKEVQ KRIAEVEEDT PIEKNLKADE NNDENKNGSK 

       790        800        810        820 
NEQLRNLLRY QTHLSKIHTL GYYSRFRVPR RVVKTAIRSW SLLLM 

References and documentation are available.

Number of amino acids: 825

Molecular weight: 93276.30

Theoretical pI: 7.32

Amino acid composition: 
Ala (A)  60	  7.3%
Arg (R)  49	  5.9%
Asn (N)  46	  5.6%
Asp (D)  51	  6.2%
Cys (C)   5	  0.6%
Gln (Q)  27	  3.3%
Glu (E)  74	  9.0%
Gly (G)  53	  6.4%
His (H)  13	  1.6%
Ile (I)  49	  5.9%
Leu (L)  74	  9.0%
Lys (K)  76	  9.2%
Met (M)  16	  1.9%
Phe (F)  36	  4.4%
Pro (P)  40	  4.8%
Ser (S)  45	  5.5%
Thr (T)  34	  4.1%
Trp (W)   7	  0.8%
Tyr (Y)  21	  2.5%
Val (V)  49	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 125
Total number of positively charged residues (Arg + Lys): 125

Atomic composition:

Carbon      C	      4146
Hydrogen    H	      6606
Nitrogen    N	      1154
Oxygen      O	      1249
Sulfur      S	        21

Formula: C4146H6606N1154O1249S21
Total number of atoms: 13176

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    70040
Abs 0.1% (=1 g/l)   0.751, assuming all pairs of Cys residues form cystines


Ext. coefficient    69790
Abs 0.1% (=1 g/l)   0.748, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is N (Asn).

The estimated half-life is: 1.4 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.51
This classifies the protein as unstable.



Aliphatic index: 82.64

Grand average of hydropathicity (GRAVY): -0.571