ProtParam

User-provided sequence:

        10         20         30         40         50         60 
NYRSYRSPLS VTSKRRVGVR ANGNKSDDNF RKDRKPLFSS IVSKAGSGAA ENLTAPPDIK 

        70         80         90        100        110        120 
KIWYSALFWS IVALLLLVGL IGYWWAISGN RGPVKVNMGF LGTIVNLPVL MTGLMTVKGL 

       130        140        150        160        170        180 
METVKNNSNF SEVQLQALYN AHSRSFIKEY ALLNQFMTLL HDQIDGLYLA IDGRPQSTTQ 

       190        200        210        220        230        240 
MYSNSLSIQN NSNNQNVNEQ EAQLNVMNTQ SKLVPGIGNT EDQCRKAFMK IVDAKGSLLR 

       250        260        270        280        290        300 
MLTMTKRRSE GIRQLMAAFD MDRTMFVSDD ISDAEYEISQ IVPAFSDTID DILAYCLWDS 

       310        320        330        340        350        360 
NVSVYDRLQR VRRRVNACHS IPDFHFTLVG SRFVVIYVNI PELYNESEKD NEFTHFCVFY 

       370        380        390        400        410        420 
YSKFLEVKER TEQMRIDEAT LPHIGFTKAI CRMEDDTPCS CDLWWTPDGG KFLEYFTQGE 

       430        440        450        460        470        480 
SILSPIDSAH VTEDSESRFF SFRSPIQYET NQLTYYARSY SSDNGEKEGE IKNRGFTVDP 

       490        500        510        520        530        540 
HLDPKDDERV SENNESVYME NVSCNKELTR SIEPTYKLAA SSGSTSASNT FGTDQDTHNY 

       550        560        570        580        590        600 
TRESHRQHNA LSLNREANSI QAYEELEDFD VGNVRTHLLR FMPFCVDEET EQSDRSAQSI 

       610        620        630        640        650        660 
ESHFSVRSDM RLPKIRRTDN GMDSKQSATR TLLTTPNVSS HTSITSYTRR KLKAQKPHTG 

       670        680        690        700        710        720 
HESEASTEFI ASVLSAKRAS TFTRASSSAE LNCRNSSTGA PLATDHGREL AANKPLSPAI 

       730        740        750        760        770        780 
SEVHIQKQHT ESKALRSPRA NSSLLAFSRL QYRKVAEAEA EGEFDMRGST SINTKEVYGG 

       790        800        810        820        830        840 
NEVGSNEGNS SSFKHRSDKT EDVKLALAEH RIPQADSYQR YNELKPYKGT KMSAATNDVS 

       850 
KSRPLDSSSD FSTSLSSM 

References and documentation are available.

Number of amino acids: 858

Molecular weight: 96683.83

Theoretical pI: 6.29

Amino acid composition: 
Ala (A)  56	  6.5%
Arg (R)  57	  6.6%
Asn (N)  53	  6.2%
Asp (D)  50	  5.8%
Cys (C)  10	  1.2%
Gln (Q)  31	  3.6%
Glu (E)  59	  6.9%
Gly (G)  40	  4.7%
His (H)  21	  2.4%
Ile (I)  39	  4.5%
Leu (L)  66	  7.7%
Lys (K)  44	  5.1%
Met (M)  22	  2.6%
Phe (F)  36	  4.2%
Pro (P)  30	  3.5%
Ser (S) 104	 12.1%
Thr (T)  57	  6.6%
Trp (W)   7	  0.8%
Tyr (Y)  30	  3.5%
Val (V)  46	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 109
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      4203
Hydrogen    H	      6600
Nitrogen    N	      1206
Oxygen      O	      1352
Sulfur      S	        32

Formula: C4203H6600N1206O1352S32
Total number of atoms: 13393

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    83825
Abs 0.1% (=1 g/l)   0.867, assuming all pairs of Cys residues form cystines


Ext. coefficient    83200
Abs 0.1% (=1 g/l)   0.861, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is N (Asn).

The estimated half-life is: 1.4 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.46
This classifies the protein as unstable.



Aliphatic index: 69.80

Grand average of hydropathicity (GRAVY): -0.601