ProtParam

User-provided sequence:

        10         20         30         40         50         60 
QHKDIIGPTE AEDEDEDDDD DDDDDDDDDD DDDDDDDDDD NNNENKDETD EEEDDDEEED 

        70         80         90        100        110        120 
EDDDEEENNK PYGGLKMVIK NVIENWDMMG GLGIDGNIDM VKESPKFVTV WSMEEKITQL 

       130        140        150        160        170        180 
QKKTMMSNFT SSVMSPALLI PYSPNWARIV STLLNDCLGL AIKSLTNATL PAVVLIDAWR 

       190        200        210        220        230        240 
RLEIHLVPDT RQKWADWEDQ DTWLQIHPPL EVVQAMNCQG PTPTVPTAPT SEYQSMKNLK 

       250        260        270        280        290        300 
ESSKIIKKTY RQEFFQTASQ TLIVQISIRD RGYIEELKKI MPKIKFVSLS GTAGFLVHLK 

       310        320        330        340        350        360 
GDDQPLRPDL NKSNERKPII SPIVSQKVPT EEPTSRVNIQ GSVNNAQDQA DEKRLTISTP 

       370        380        390        400        410        420 
AKATVKNNER SIPTSTAMIK DPKMTDVIKL ASDKKKPDYD KMENVLPSGS LQTTRSTNTH 

       430        440        450        460        470        480 
NPIVAHNGPI SNSVPAPNSN PSKTRESDNK LQTLSDQKQQ QKLDSFTVAP VRKLGPEPTN 

       490        500        510        520        530        540 
SVVAGSTGTA NMISKTTTTT ANSLTPSISQ RGSVPSNIIS DKGKADEHDL LIEKQGRSFR 

       550        560 
PCEISQNHDA DQKGSTSAVA TM 

References and documentation are available.

Number of amino acids: 562

Molecular weight: 62478.15

Theoretical pI: 4.56

Amino acid composition: 
Ala (A)  28	  5.0%
Arg (R)  18	  3.2%
Asn (N)  35	  6.2%
Asp (D)  64	 11.4%
Cys (C)   3	  0.5%
Gln (Q)  28	  5.0%
Glu (E)  39	  6.9%
Gly (G)  23	  4.1%
His (H)   8	  1.4%
Ile (I)  37	  6.6%
Leu (L)  35	  6.2%
Lys (K)  45	  8.0%
Met (M)  16	  2.8%
Phe (F)   8	  1.4%
Pro (P)  37	  6.6%
Ser (S)  49	  8.7%
Thr (T)  45	  8.0%
Trp (W)   7	  1.2%
Tyr (Y)   6	  1.1%
Val (V)  31	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2678
Hydrogen    H	      4295
Nitrogen    N	       747
Oxygen      O	       932
Sulfur      S	        19

Formula: C2678H4295N747O932S19
Total number of atoms: 8671

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47565
Abs 0.1% (=1 g/l)   0.761, assuming all pairs of Cys residues form cystines


Ext. coefficient    47440
Abs 0.1% (=1 g/l)   0.759, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is Q (Gln).

The estimated half-life is: 0.8 hours (mammalian reticulocytes, in vitro).
                            10 min (yeast, in vivo).
                            10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.98
This classifies the protein as unstable.



Aliphatic index: 70.94

Grand average of hydropathicity (GRAVY): -0.847