ProtParam

User-provided sequence:

        10         20         30         40         50         60 
QQTTGHETTI KFARLSGRSA VYLTAYGKRK LEESDSAYTY DLGCIDGMPF YVDCVGCYTD 

        70         80         90        100        110        120 
SSEGDAGYMP ADFLNRNCRC EPSPNVPDQV EWGLIYEQRF SYIGRYETLR LLSILGLQSA 

       130        140        150        160        170        180 
VAICNLEKIH CVFNIRNLAQ LLSKSACTVV GDLPFIEHHT ASIIILPNAK MLVLRHATTL 

       190        200        210        220        230        240 
FFFEDHMESL QYGLPSDESG PVASELLRKV NRVYNHFGWS KQSESKLHAR QETDRYRRQF 

       250        260        270        280        290        300 
STLSSGSEEN EPDSNVDDEE TSNLQLSCIE PDKLVTNLDK GFLTRWDAAA DTNYWLFNGS 

       310        320        330        340        350        360 
EINPIHNVSS LSKFEKSTRL QRQKDMKLNR ISRNFERSID EIDNELDTLK YTSPECIKRG 

       370        380        390        400        410        420 
STNQYQSTKN RGSPTILQPF TNIIDKYKSF GRETARYHKY GSDEWFTWQH LHQECSTNKT 

       430        440        450        460        470        480 
VADNMDDLDG AHLILAFDER SYEYKISGTT TYNRDFSTFR LPIVEDPIGA SSTLRLDTVP 

       490        500        510        520        530        540 
VQFQNLLTGI GFNGSLYAND CYYGLLFDDR CISYLMSEKF GDQYNLNLLE FNDVKAFDKK 

       550        560        570        580        590        600 
TLPTITWLDD GFFQTDLFST LITCDHEKLK VTVVNGFISC YSLTIYGVSD KSNPVFNVCP 

       610        620        630        640        650        660 
VNHSSPLDLP RIYRREKKVD KDFAFVTVGP KNHGVAIYII GNIDIVDVSW CSEEVRLVMY 

       670        680        690        700        710        720 
PDVNIYRERS NDKGGLIDST FKNFIKIVKN IEWIYVIGND GCALLFDQHM FNCIKMFNIS 

       730        740        750        760        770        780 
CLEPISNFQT DKFKVYSSNR FNRVDGDDPI IVPLLAHCIE LSSVNLLIDF NHVEVCQGAA 

       790        800        810        820        830        840 
LFSYSGYTVF LNNKFQLGGY AFRNTYQFPA FELPIEQVLL KEDQPLLLEA PNLLRSPGWV 

       850        860        870        880        890        900 
PELNSFASKT DELFAKKFSY APNPIKWSSD HEELNHLREK IFEVTKPHFS NCSSNPWKLQ 

       910        920        930        940        950 
YYKKLTIIEN TINWGGSALF VESDFCERTS VARQIIARSK LWRTFLDDNE NPIQPPM 

References and documentation are available.

Number of amino acids: 957

Molecular weight: 109714.85

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)  40	  4.2%
Arg (R)  46	  4.8%
Asn (N)  63	  6.6%
Asp (D)  65	  6.8%
Cys (C)  24	  2.5%
Gln (Q)  32	  3.3%
Glu (E)  59	  6.2%
Gly (G)  48	  5.0%
His (H)  22	  2.3%
Ile (I)  61	  6.4%
Leu (L)  92	  9.6%
Lys (K)  53	  5.5%
Met (M)  11	  1.1%
Phe (F)  57	  6.0%
Pro (P)  41	  4.3%
Ser (S)  81	  8.5%
Thr (T)  55	  5.7%
Trp (W)  14	  1.5%
Tyr (Y)  42	  4.4%
Val (V)  51	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 124
Total number of positively charged residues (Arg + Lys): 99

Atomic composition:

Carbon      C	      4922
Hydrogen    H	      7529
Nitrogen    N	      1301
Oxygen      O	      1479
Sulfur      S	        35

Formula: C4922H7529N1301O1479S35
Total number of atoms: 15266

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   141080
Abs 0.1% (=1 g/l)   1.286, assuming all pairs of Cys residues form cystines


Ext. coefficient   139580
Abs 0.1% (=1 g/l)   1.272, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is Q (Gln).

The estimated half-life is: 0.8 hours (mammalian reticulocytes, in vitro).
                            10 min (yeast, in vivo).
                            10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.91
This classifies the protein as unstable.



Aliphatic index: 81.99

Grand average of hydropathicity (GRAVY): -0.371