ProtParam

User-provided sequence:

        10         20         30         40         50         60 
REDVAVSVNS DFRVSTKRVN GQTDVTEPID LEFEKGNKLI EKLDPQAERM SSKRNGTSKK 

        70         80         90        100        110        120 
SGDESSDNSD IKEVGLNADA NKKELNPVRE FTSLENQCRD CQCLFGWNVR LERRRLRVGH 

       130        140        150        160        170        180 
LPNVYTIRIQ EGKKIPKRAH LRLEEHEEVQ EIYANPECDH NIFSIWHYVQ GNYQNINFTG 

       190        200        210        220        230        240 
IMTLFKEFDI EESAKPFAGC FLEYCKEWVT EDDINKEVGK KTGNDSEEET HVTTGNLLSF 

       250        260        270        280        290        300 
TSGIGSADRL KIRERQSISA LKQWQEKVEG TTDLLMSGYI LGVSFAATFC YKECYNVFRK 

       310        320        330        340        350        360 
WNGAHLIDIR NSRWSHYLLE HLSAHAKKCK ESCWIAKCVE CDLYHVMIKH QTLDYLAKGC 

       370        380        390        400        410        420 
RACAKGNSIL FLKDLPPIYV IPKNEKLIIQ GKSFDRKAFL GRGHEDDIFK VEVKADSDLA 

       430        440        450        460        470        480 
KPFHIKDKYL SVTELDTDYL HHENLIKRFR SASLKWSQNR ETIRDYLHQI TADETGPEEK 

       490        500        510        520 
WLLVVDDCIE EETPTIKRFD SGNETGNHVA SQDSDNLTGI KITLTM 

References and documentation are available.

Number of amino acids: 526

Molecular weight: 60547.35

Theoretical pI: 6.03

Amino acid composition: 
Ala (A)  25	  4.8%
Arg (R)  29	  5.5%
Asn (N)  29	  5.5%
Asp (D)  36	  6.8%
Cys (C)  15	  2.9%
Gln (Q)  16	  3.0%
Glu (E)  49	  9.3%
Gly (G)  29	  5.5%
His (H)  18	  3.4%
Ile (I)  35	  6.7%
Leu (L)  44	  8.4%
Lys (K)  46	  8.7%
Met (M)   5	  1.0%
Phe (F)  21	  4.0%
Pro (P)  13	  2.5%
Ser (S)  35	  6.7%
Thr (T)  29	  5.5%
Trp (W)   9	  1.7%
Tyr (Y)  15	  2.9%
Val (V)  28	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 85
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      2669
Hydrogen    H	      4189
Nitrogen    N	       749
Oxygen      O	       821
Sulfur      S	        20

Formula: C2669H4189N749O821S20
Total number of atoms: 8448

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72725
Abs 0.1% (=1 g/l)   1.201, assuming all pairs of Cys residues form cystines


Ext. coefficient    71850
Abs 0.1% (=1 g/l)   1.187, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is R (Arg).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
                            2 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.01
This classifies the protein as unstable.



Aliphatic index: 78.76

Grand average of hydropathicity (GRAVY): -0.642