ProtParam

User-provided sequence:

        10         20         30         40         50         60 
RIAQQVIDAK EIAGNKSEEF DEFYLERTIL LYSILGVFAF FSAYVIRRQF SVEKELRELH 

        70         80         90        100        110        120 
LLHRNTFKDN ELKQEELIRL SQDKLQNAFN NLLDLNSMMT ENFITVLHSF KAEHAMELQE 

       130        140        150        160        170        180 
FSKSLLKSQE EIYSVTLSSN TELQSLRKMI NKFISEEPIV PVASSSSSSI MQKSPCFSAN 

       190        200        210        220        230        240 
LVKFFEDFKL PILKSTCSLA GALDSLNSTN PTELRTEINN CRDQINQENL NNINNTGISQ 

       250        260        270        280        290        300 
EKDEQAKLQD IKFEDMMSKG YVKILSIPCY FEDEYHSLIE IKLYSAWIQP NHIQFKQLER 

       310        320        330        340        350        360 
SNRAEFEGLI TWENKVMPIR DSVSVRFRKF TSSFFEYNAI EIEEVLIDEC LEIVIFQQPA 

       370        380        390        400        410        420 
SCPNLLYKDK SEILTSTAGI AEPNSKVITA ACDLSAYNFK KKYVKEIDKE ENFGKQNEER 

       430        440        450        460        470        480 
ESWENKNTLF GLDIELEEGI CEKEKYCSPD VPERVRSLHR EPGSSHEKSK VKKWEDFSLF 

       490        500        510        520        530        540 
SNSSDGPNRP TAPQTLADQE KREGNERIDA QRIDVKSAQR FDHTRLSGTS LPSSHEKQVN 

       550        560        570        580 
TTSGTTIVTT DHLGIKANQA TKNRDLAFSG IISVWLILGY ILLRNAM 

References and documentation are available.

Number of amino acids: 587

Molecular weight: 67452.23

Theoretical pI: 5.36

Amino acid composition: 
Ala (A)  30	  5.1%
Arg (R)  27	  4.6%
Asn (N)  39	  6.6%
Asp (D)  27	  4.6%
Cys (C)   9	  1.5%
Gln (Q)  27	  4.6%
Glu (E)  62	 10.6%
Gly (G)  18	  3.1%
His (H)  11	  1.9%
Ile (I)  46	  7.8%
Leu (L)  56	  9.5%
Lys (K)  45	  7.7%
Met (M)   9	  1.5%
Phe (F)  32	  5.5%
Pro (P)  18	  3.1%
Ser (S)  60	 10.2%
Thr (T)  28	  4.8%
Trp (W)   5	  0.9%
Tyr (Y)  14	  2.4%
Val (V)  24	  4.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2988
Hydrogen    H	      4716
Nitrogen    N	       806
Oxygen      O	       934
Sulfur      S	        18

Formula: C2988H4716N806O934S18
Total number of atoms: 9462

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48860
Abs 0.1% (=1 g/l)   0.724, assuming all pairs of Cys residues form cystines


Ext. coefficient    48360
Abs 0.1% (=1 g/l)   0.717, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is R (Arg).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
                            2 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.89
This classifies the protein as unstable.



Aliphatic index: 84.74

Grand average of hydropathicity (GRAVY): -0.506