ProtParam
User-provided sequence:
10 20 30 40 50 60
SKVKKKVQKK RLIILRVDKL YVKSRCLACH GFQRCTRSSN VMQFLCPCCF VHGCLTMLAT
70 80 90 100 110 120
EPPEFCIPCR YDKAAGYEKP AEKTEEQFDD DSIQLVQQEE ADLDIADATL DVTQEKSNTG
130 140 150 160 170 180
PKTTPPAADL MPSASPTNAT QSLVDMTHLE EVNNTLTINN GSSAEPEETH MTERDHNGSD
190 200 210 220 230 240
NNSTNHDSGP DEELARFLSL DDDGDEESTN ELAREGIIEV VAEPSDHRND TVINQTRSVE
250 260 270
NEEEEDNQRV SELRVRKIRL NEGEPNQNDP RRAM
References and
documentation are available.
Number of amino acids: 274
Molecular weight: 30763.88
Theoretical pI: 4.66
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1292
Hydrogen H 2074
Nitrogen N 384
Oxygen O 456
Sulfur S 15
Formula: C
1292H
2074N
384O
456S
15
Total number of atoms: 4221
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4970
Abs 0.1% (=1 g/l) 0.162, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.145, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is S (Ser).
The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.52
This classifies the protein as unstable.
Aliphatic index: 65.84
Grand average of hydropathicity (GRAVY): -0.885