ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SRERTLFKFL KYGGISLMGL TLSELFKLIR TQKGARELAR SYSIDSRFSI MTYLPIWKDK 

        70         80         90        100        110        120 
MIISFLADLK KRLLFRKSTV DHSMEKYNRK ALETISVLDK HRTQTYETFA RSRDGSHKKL 

       130        140        150        160        170        180 
LAMLIRVDEF GCNMGQGYFP VMAHAADGLL IAKGGPVDYP KCNVCVLSEK PYTIFRKVAD 

       190        200        210        220        230        240 
DLDMINIIDP FNEILFERVR SKSTILDSIQ DKSGFFTSTF SGDSNALAIL MFKHRPWIHL 

       250        260        270        280        290        300 
HDPAIAFNGG YNPKFEETPP IYLEIYRLNM YEQSFDMEVK RQMQSRTASY AGDCGIVFDY 

       310        320        330        340        350        360 
KETHPTKLDE GIAFHCIQKD DTWEIKVLKH GFKLETTSKE LEDLLSNNLV SRNISNIAEG 

       370        380        390        400        410        420 
HLGYLQSEQR GKLDHIMRGK MPIMDQLIHE CADPDISKLA DIGRASIALN ISKLNKNKTT 

       430        440        450        460 
DLRPDQRFDY LTVNYGEKSF ALAALCGVLG AGIIAVSESM 

References and documentation are available.

Number of amino acids: 460

Molecular weight: 52429.50

Theoretical pI: 8.60

Amino acid composition: 
Ala (A)  28	  6.1%
Arg (R)  26	  5.7%
Asn (N)  17	  3.7%
Asp (D)  32	  7.0%
Cys (C)   7	  1.5%
Gln (Q)  12	  2.6%
Glu (E)  26	  5.7%
Gly (G)  28	  6.1%
His (H)  13	  2.8%
Ile (I)  37	  8.0%
Leu (L)  48	 10.4%
Lys (K)  37	  8.0%
Met (M)  16	  3.5%
Phe (F)  24	  5.2%
Pro (P)  15	  3.3%
Ser (S)  35	  7.6%
Thr (T)  23	  5.0%
Trp (W)   3	  0.7%
Tyr (Y)  17	  3.7%
Val (V)  16	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 63

Atomic composition:

Carbon      C	      2347
Hydrogen    H	      3711
Nitrogen    N	       633
Oxygen      O	       681
Sulfur      S	        23

Formula: C2347H3711N633O681S23
Total number of atoms: 7395

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42205
Abs 0.1% (=1 g/l)   0.805, assuming all pairs of Cys residues form cystines


Ext. coefficient    41830
Abs 0.1% (=1 g/l)   0.798, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.40
This classifies the protein as stable.



Aliphatic index: 88.24

Grand average of hydropathicity (GRAVY): -0.282