ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SSCSAQMMFK ADDQHSLDAW KTELEPRLTV DWKTRDYPNV SMLYLKKLAS TDLESSPDQL 

        70         80         90        100        110        120 
GDRQSYMLDD VVEELVWSRP VFLPNAKKAL SARAATETPP VLTLYDQTWN RIEQLKENLL 

       130        140        150        160        170        180 
RLEGNADFVK EGAAFAEETE AMQQLQKSNF FMGLYEEDFA GFLTATDSKD KIFFPGKYNQ 

       190        200        210        220        230        240 
LHIFFNNYDV KTAQLIDLLP EVIVDCFEKA KEAAHEIDKL NTSSMCKELD LDVGLRQSMI 

       250        260        270        280        290        300 
QAYRTTFVYK YENASLEVLK QVREIMTKEL ADDVSNLEGK LLLELDRAEP GLLCALDKAF 

       310        320        330        340        350        360 
QQLNWWIISP QNAFSYRKAT DDHNPTFSPE FKDLFAFPGY DITLGMISTN DTNLVGNAFG 

       370        380        390        400        410        420 
YAQWHAVTNA NLSVVHRFFE DYKSLTSIND GTMTSVETED KEVQEDIKTE TDPFFDRKFI 

       430        440        450        460        470        480 
NFDPKGEFQT GGAFLEEICY DSLQILGKLD HRWRFLNFNG LRIWSPAFRC VVACPEQNRR 

       490        500        510        520        530        540 
QAKTGPLLTL QMARTSPIGI SHLAESMIFE RISSRLVAKG DAFRSFPTMG AGKLQFTQWQ 

       550        560        570        580        590        600 
GSCKDKLDFL NVVRGDGLQA AFSGFQFGSY VTSYPFISDS YYVKEGSLIK ELDQDQVLNF 

       610        620        630        640        650        660 
EDLANQSLLL PKYHPKKTPL CFAFHAGQSV MRPTHFLKLR VPDTANYQQV VNIAEQISSL 

       670        680 
STDATLKKIF HSAASNKLAK IITRKEGM 

References and documentation are available.

Number of amino acids: 688

Molecular weight: 78312.86

Theoretical pI: 5.26

Amino acid composition: 
Ala (A)  53	  7.7%
Arg (R)  28	  4.1%
Asn (N)  31	  4.5%
Asp (D)  49	  7.1%
Cys (C)   9	  1.3%
Gln (Q)  36	  5.2%
Glu (E)  45	  6.5%
Gly (G)  32	  4.7%
His (H)  12	  1.7%
Ile (I)  29	  4.2%
Leu (L)  74	 10.8%
Lys (K)  47	  6.8%
Met (M)  16	  2.3%
Phe (F)  46	  6.7%
Pro (P)  27	  3.9%
Ser (S)  48	  7.0%
Thr (T)  41	  6.0%
Trp (W)  10	  1.5%
Tyr (Y)  21	  3.1%
Val (V)  34	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 75

Atomic composition:

Carbon      C	      3521
Hydrogen    H	      5428
Nitrogen    N	       920
Oxygen      O	      1054
Sulfur      S	        25

Formula: C3521H5428N920O1054S25
Total number of atoms: 10948

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    86790
Abs 0.1% (=1 g/l)   1.108, assuming all pairs of Cys residues form cystines


Ext. coefficient    86290
Abs 0.1% (=1 g/l)   1.102, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.74
This classifies the protein as stable.



Aliphatic index: 80.42

Grand average of hydropathicity (GRAVY): -0.347