ProtParam

User-provided sequence:

        10         20         30         40         50         60 
TNQVTKHQGW NTKIVVNRSD NRKWFDILTI ATKIPSDEQI FHGSDQFVVL QYKGQMQGVI 

        70         80         90        100        110        120 
LEKDLNENGA LILLKSVPLG VFSHSLDTFW TDWFPSFTKL NTIRVVKGSK LPAFLAPIAI 

       130        140        150        160        170        180 
EASSRLRSLA HQVHWDVADN ISEFVNPTNQ LFHEVKNLAM IAAEEVIDLM TIGLIKKQLE 

       190        200        210        220        230        240 
TSLKPYAFTC ISGGLSHGIL IVSVKQLPEQ SIKSKFWYNL LSVFDKIFSD RDFCIPADAK 

       250        260        270        280        290        300 
KFKTEAHGRA DFAFCGCRGE LKTNLEKALN AFSLGSSGAG HHFIFIPIST TNSLLSTPLT 

       310        320        330        340        350        360 
YYTNVDLDRD PLSVLEKNDF FDSWTPLSSK EKSSIRDTTS GTKGTYKRNR NSQGNFSPLS 

       370        380        390        400 
GLADGDNEDD EYAEQFTADL VNKAREFQSL AIKRRLDDSM 

References and documentation are available.

Number of amino acids: 400

Molecular weight: 44887.94

Theoretical pI: 6.55

Amino acid composition: 
Ala (A)  25	  6.2%
Arg (R)  15	  3.8%
Asn (N)  22	  5.5%
Asp (D)  28	  7.0%
Cys (C)   4	  1.0%
Gln (Q)  15	  3.8%
Glu (E)  19	  4.8%
Gly (G)  22	  5.5%
His (H)  10	  2.5%
Ile (I)  26	  6.5%
Leu (L)  41	 10.2%
Lys (K)  30	  7.5%
Met (M)   4	  1.0%
Phe (F)  26	  6.5%
Pro (P)  14	  3.5%
Ser (S)  38	  9.5%
Thr (T)  26	  6.5%
Trp (W)   7	  1.8%
Tyr (Y)   7	  1.8%
Val (V)  21	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      2020
Hydrogen    H	      3147
Nitrogen    N	       539
Oxygen      O	       603
Sulfur      S	         8

Formula: C2020H3147N539O603S8
Total number of atoms: 6317

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    49180
Abs 0.1% (=1 g/l)   1.096, assuming all pairs of Cys residues form cystines


Ext. coefficient    48930
Abs 0.1% (=1 g/l)   1.090, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is T (Thr).

The estimated half-life is: 7.2 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 25.49
This classifies the protein as stable.



Aliphatic index: 86.80

Grand average of hydropathicity (GRAVY): -0.273