ProtParam
User-provided sequence:
10 20 30 40 50 60
WKLLDDKAYL YDDFIFEVED TEANYGGVRI SEHNKVIIDC GKEKLFDLDF TTAIGIIKCG
70 80 90 100 110 120
AAKGAAIGAP ADEFVVVKSK SPDQENIPYG LGNRGKLYPE PHPKGQKVDN ATIFYKPRRI
130 140 150 160 170 180
GLHEFWKQAM DRTGSTAVAW KEKPLANLAN CLKVAGPVEI SKEGYKVPIE AELKNVYEEN
190 200 210 220 230 240
AFDPAFKAIA DFTRWGHSVQ IVHEADFYPK DKGFDRWFAA IAPQSIIITG DVDFLAANVK
250
LSLPKTTLGM
References and
documentation are available.
Number of amino acids: 250
Molecular weight: 27813.77
Theoretical pI: 5.81
Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1272
Hydrogen H 1954
Nitrogen N 326
Oxygen O 365
Sulfur S 5
Formula: C
1272H
1954N
326O
365S
5
Total number of atoms: 3922
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41035
Abs 0.1% (=1 g/l) 1.475, assuming all pairs of Cys residues form cystines
Ext. coefficient 40910
Abs 0.1% (=1 g/l) 1.471, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is W (Trp).
The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro).
3 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 27.95
This classifies the protein as stable.
Aliphatic index: 83.96
Grand average of hydropathicity (GRAVY): -0.282