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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
WKLLDDKAYL YDDFIFEVED TEANYGGVRI SEHNKVIIDC GKEKLFDLDF TTAIGIIKCG

70 80 90 100 110 120
AAKGAAIGAP ADEFVVVKSK SPDQENIPYG LGNRGKLYPE PHPKGQKVDN ATIFYKPRRI

130 140 150 160 170 180
GLHEFWKQAM DRTGSTAVAW KEKPLANLAN CLKVAGPVEI SKEGYKVPIE AELKNVYEEN

190 200 210 220 230 240
AFDPAFKAIA DFTRWGHSVQ IVHEADFYPK DKGFDRWFAA IAPQSIIITG DVDFLAANVK

250
LSLPKTTLGM


References and documentation are available.

Number of amino acids: 250 Molecular weight: 27813.77 Theoretical pI: 5.81
Amino acid composition: 
Ala (A) 27 10.8% Arg (R) 7 2.8% Asn (N) 10 4.0% Asp (D) 19 7.6% Cys (C) 3 1.2% Gln (Q) 5 2.0% Glu (E) 17 6.8% Gly (G) 19 7.6% His (H) 5 2.0% Ile (I) 19 7.6% Leu (L) 16 6.4% Lys (K) 25 10.0% Met (M) 2 0.8% Phe (F) 14 5.6% Pro (P) 14 5.6% Ser (S) 8 3.2% Thr (T) 10 4.0% Trp (W) 5 2.0% Tyr (Y) 9 3.6% Val (V) 16 6.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 36 Total number of positively charged residues (Arg + Lys): 32 Atomic composition: Carbon C 1272 Hydrogen H 1954 Nitrogen N 326 Oxygen O 365 Sulfur S 5 Formula: C1272H1954N326O365S5 Total number of atoms: 3922 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 41035 Abs 0.1% (=1 g/l) 1.475, assuming all pairs of Cys residues form cystines Ext. coefficient 40910 Abs 0.1% (=1 g/l) 1.471, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is W (Trp). The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro). 3 min (yeast, in vivo). 2 min (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 27.95 This classifies the protein as stable. Aliphatic index: 83.96 Grand average of hydropathicity (GRAVY): -0.282