ProtParam

User-provided sequence:

        10         20         30         40         50         60 
YDDDGGYQNR GGRNSSGYSD DNNSSGYNSS GYSNKNRNSS GYSDDNNSGY SDNNNSSGYS 

        70         80         90        100        110        120 
DDNNSGYSSK KKNSSGYSDD NNSGYSDNNS SGYSSKKKNS SGYSDDNNSG YSDDNNSGYS 

       130        140        150        160        170        180 
SKKKNSSGYS DDNNSSGYSD DNSSSGYSSK NKNSSGYSDD NNSGYSDNNN SSGYSDDNNS 

       190        200        210        220        230        240 
GYSSKKKNSS GYSDDNNSGY SDNNNSGYSD DNNSGYSDNN SSGYSSKKKN SSGYSDDNNS 

       250        260        270        280        290        300 
GYSDDNNSGY SSKNKNSSGY SDDNNSGYSD NNNSGYSDNN NSGYSDNNNS SGYSDDNNSG 

       310        320        330        340        350        360 
YSSKKKNSSG YSDDNNSGYS DNNNSGYSDN NNSGYSDNNN SGYSDNNNSG YSDNNNSDND 

       370        380        390        400        410        420 
NISTNSFQQR VKSEMKDFRD EGIKSKFQNV RDEGIMNEAK TVYDGQNNNG SDNNNNNSNA 

       430 
FQKVKDFLGM 

References and documentation are available.

Number of amino acids: 430

Molecular weight: 46233.15

Theoretical pI: 4.22

Amino acid composition: 
Ala (A)   2	  0.5%
Arg (R)   6	  1.4%
Asn (N) 100	 23.3%
Asp (D)  58	 13.5%
Cys (C)   0	  0.0%
Gln (Q)   6	  1.4%
Glu (E)   4	  0.9%
Gly (G)  49	 11.4%
His (H)   0	  0.0%
Ile (I)   3	  0.7%
Leu (L)   1	  0.2%
Lys (K)  30	  7.0%
Met (M)   3	  0.7%
Phe (F)   5	  1.2%
Pro (P)   0	  0.0%
Ser (S) 114	 26.5%
Thr (T)   2	  0.5%
Trp (W)   0	  0.0%
Tyr (Y)  43	 10.0%
Val (V)   4	  0.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1843
Hydrogen    H	      2680
Nitrogen    N	       584
Oxygen      O	       820
Sulfur      S	         3

Formula: C1843H2680N584O820S3
Total number of atoms: 5930

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64070
Abs 0.1% (=1 g/l)   1.386

Estimated half-life:

The N-terminal of the sequence considered is Y (Tyr).

The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro).
                            10 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 17.90
This classifies the protein as stable.



Aliphatic index: 6.79

Grand average of hydropathicity (GRAVY): -1.960