ProtParam
User-provided sequence:
10 20 30 40 50 60
YFTSPRKYSF TKTSKQVRHP LPFKLDKTLF QKVYQRKVDA TKEIEAVKLI HISRFRARHR
70 80 90 100 110 120
AAMDQYLTEV AAVRSVDRIE KYMNHTGSRS DYRVWVGFNK VKTPHAENIQ NISVIEGSAK
130 140 150 160 170
KVKHLKQLFY WYRSKAIVEN SAFIRMRFLK PEPVSETPLR RGIVQYEKFH ALYM
References and
documentation are available.
Number of amino acids: 174
Molecular weight: 20713.10
Theoretical pI: 10.26
Total number of negatively charged residues (Asp + Glu): 15
Total number of positively charged residues (Arg + Lys): 35
Atomic composition:
Carbon C 945
Hydrogen H 1487
Nitrogen N 269
Oxygen O 248
Sulfur S 4
Formula: C
945H
1487N
269O
248S
4
Total number of atoms: 2953
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25900
Abs 0.1% (=1 g/l) 1.250
Estimated half-life:
The N-terminal of the sequence considered is Y (Tyr).
The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.55
This classifies the protein as unstable.
Aliphatic index: 76.72
Grand average of hydropathicity (GRAVY): -0.594