ProtParam

User-provided sequence:

        10         20         30         40         50         60 
AEGVFQGAIG IDLGTTYSCV ATYESSVEII ANEQGNRVTP SFVAFTPQER LIGDAAKNQA 

        70         80         90        100        110        120 
ALNPRNTVFD AKRLIGRRFD DESVQKDMKT WPFKVIDVDG NPVIEVQYLE ETKTFSPQEI 

       130        140        150        160        170        180 
SAMVLTKMKE IAEAKIGKKV EKAVITVPAY FNDAQRQATK DAGAISGLNV LRIINEPTAA 

       190        200        210        220        230        240 
AIAYGLGAGK SEKERHVLIF DLGGGTFDVS LLHIAGGVYT VKSTSGNTHL GGQDFDTNLL 

       250        260        270        280        290        300 
EHFKAEFKKK TGLDISDDAR ALRRLRTAAE RAKRTLSSVT QTTVEVDSLF DGEDFESSLT 

       310        320        330        340        350        360 
RARFEDLNAA LFKSTLEPVE QVLKDAKISK SQIDEVVLVG GSTRIPKVQK LLSDFFDGKQ 

       370        380        390        400        410        420 
LEKSINPDEA VAYGAAVQGA ILTGQSTSDE TKDLLLLDVA PLSLGVGMQG DIFGIVVPRN 

       430        440        450        460        470        480 
TTVPTIKRRT FTTVSDNQTT VQFPVYQGER VNCKENTLLG EFDLKNIPMM PAGEPVLEAI 

       490        500        510        520        530        540 
FEVDANGILK VTAVEKSTGK SSNITISNAV GRLSSEEIEK MVNQAEEFKA ADEAFAKKHE 

       550        560        570        580        590        600 
ARQRLESYVA SIEQTVTDPV LSSKLKRGSK SKIEAALSDA LAALQIEDPS ADELRKAEVG 

       610 
LKRVVTKAMS SR 

References and documentation are available.

Number of amino acids: 612

Molecular weight: 66463.38

Theoretical pI: 5.37

Amino acid composition: 
Ala (A)  61	 10.0%
Arg (R)  29	  4.7%
Asn (N)  22	  3.6%
Asp (D)  38	  6.2%
Cys (C)   2	  0.3%
Gln (Q)  25	  4.1%
Glu (E)  49	  8.0%
Gly (G)  42	  6.9%
His (H)   5	  0.8%
Ile (I)  36	  5.9%
Leu (L)  51	  8.3%
Lys (K)  47	  7.7%
Met (M)   8	  1.3%
Phe (F)  26	  4.2%
Pro (P)  20	  3.3%
Ser (S)  44	  7.2%
Thr (T)  45	  7.4%
Trp (W)   1	  0.2%
Tyr (Y)   9	  1.5%
Val (V)  52	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 87
Total number of positively charged residues (Arg + Lys): 76

Atomic composition:

Carbon      C	      2929
Hydrogen    H	      4752
Nitrogen    N	       804
Oxygen      O	       932
Sulfur      S	        10

Formula: C2929H4752N804O932S10
Total number of atoms: 9427

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19035
Abs 0.1% (=1 g/l)   0.286, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.285, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.99
This classifies the protein as stable.



Aliphatic index: 90.05

Grand average of hydropathicity (GRAVY): -0.245