ProtParam
User-provided sequence:
10 20 30 40 50 60
APLSLANSLT NLLSSSNGNL SVPQTPTKEH HPTAPTSNRK CDLPRSNSTT ISQLTKDRLK
70 80 90 100 110 120
NMIANRSKGE SNSQSNLMSN SVTANGNGHD NGRKLKNSNS QVNVSSPHFE PYRLPTSLAN
130 140 150 160 170 180
AHNLQQASEF QLRKVNSEPN LKMRIRAKLL SKGSSPVQHV QQNNSQFNFT HPQLKRSDSE
190 200 210 220 230 240
TSQNVPLDFM QSSSQTNLPH LMLPSPSLPN LAAAGAFHGL NLPVGQDLNA FMAVANLSPF
250 260 270 280 290 300
LSLPSLLNKK LELGGLTDEG DRNGLIGSSS TSSLASNVSM GSHQYQSLLK QQIRDLVLRR
310 320 330 340 350 360
KSLVREDPEG EGLAELYNGL LPQAKLQQLQ ALAAESGFLA KQEPTCTTGL GYDQAMVRHE
370 380 390 400 410 420
CCCGNNASHV ENGGRIQSIW SKLIEHGHVQ KCEKVTAKKA SLEQLQLVHS QTYTTFFAVS
430 440 450 460 470 480
PTACLKIDAN SLPLKRFLQL PCGGIGVDSD TYFNDASTQT AARLAAGTLI ELSSQVAEGR
490 500 510 520 530 540
LKNGFACIRP PGHHAEHEQA MGFCFFNNVA VAVKVLQTKY PAQCAKIAII DWDVHHGNGT
550 560 570 580 590 600
QLSFENDPNV LYMSLHRHDK GNFFPGTGSV TEVGKNDAKG LTVNVPFSGD VMRDPEYLAA
610 620 630 640 650 660
WRTVIEPVMA SFCPDFIIVS AGFDACHGHP NALGGYEVTP EMFGYMTKSL LNYASGKVVL
670 680 690 700 710 720
ALEGGYDLKS ISEAAQQCVQ ALIGESDDAG RLSSVALESL PNPSAVETLQ KVIAIHKSYW
730 740 750
PALHGQEAAI NTTEMQWRNL KLQVQMQQQQ QQQQT
References and
documentation are available.
Number of amino acids: 755
Molecular weight: 82049.54
Theoretical pI: 7.71
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 3578
Hydrogen H 5683
Nitrogen N 1035
Oxygen O 1118
Sulfur S 30
Formula: C
3578H
5683N
1035O
1118S
30
Total number of atoms: 11444
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49235
Abs 0.1% (=1 g/l) 0.600, assuming all pairs of Cys residues form cystines
Ext. coefficient 48360
Abs 0.1% (=1 g/l) 0.589, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is A (Ala).
The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.20
This classifies the protein as unstable.
Aliphatic index: 80.65
Grand average of hydropathicity (GRAVY): -0.375