ProtParam

User-provided sequence:

        10         20         30         40 
CGEGRVTCLF EDVVPMNYMV YYNFFAFVLL PLHILSLARG SA 

References and documentation are available.

Number of amino acids: 42

Molecular weight: 4757.62

Theoretical pI: 5.44

Amino acid composition: 
Ala (A)   3	  7.1%
Arg (R)   2	  4.8%
Asn (N)   2	  4.8%
Asp (D)   1	  2.4%
Cys (C)   2	  4.8%
Gln (Q)   0	  0.0%
Glu (E)   2	  4.8%
Gly (G)   3	  7.1%
His (H)   1	  2.4%
Ile (I)   1	  2.4%
Leu (L)   6	 14.3%
Lys (K)   0	  0.0%
Met (M)   2	  4.8%
Phe (F)   4	  9.5%
Pro (P)   2	  4.8%
Ser (S)   2	  4.8%
Thr (T)   1	  2.4%
Trp (W)   0	  0.0%
Tyr (Y)   3	  7.1%
Val (V)   5	 11.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 3
Total number of positively charged residues (Arg + Lys): 2

Atomic composition:

Carbon      C	       221
Hydrogen    H	       332
Nitrogen    N	        52
Oxygen      O	        57
Sulfur      S	         4

Formula: C221H332N52O57S4
Total number of atoms: 666

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4595
Abs 0.1% (=1 g/l)   0.966, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.940, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is C (Cys).

The estimated half-life is: 1.2 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.55
This classifies the protein as unstable.



Aliphatic index: 106.67

Grand average of hydropathicity (GRAVY): 0.795