ProtParam

User-provided sequence:

        10         20         30         40         50         60 
DPGFMSLDEY GSSPGDLRAF SSHRSNTPES IAETPPARDG SGGELYGYMS MDRPLSHCGR 

        70         80         90        100        110        120 
PYRRSSGDGA QDLDRGLRKR TYSLTTPARQ RQVPQPSSAS LDEYTLMRAT FSGSSGRLCP 

       130        140        150        160        170        180 
SLPASSPKVA YNPYPEDYGD IEIGSHKSSS SNLGADDGYM PMTPGAALRS GGPNSCKSDD 

       190        200        210        220        230        240 
YMPMSPTSVS APKQILQPRS AAALPPSGAA VPAPPSGAGR TFPVNGGGYK ASSPAESSPE 

       250        260        270        280        290        300 
DSGYMRMWCG SKLSMENPDP KLLPNGDYLN MSPSEAGTAG TPPDFFSAAL RPGGEALKGV 

       310        320        330 
PGHCYSSLPR SYKAPCTCGG GDNDQYVLMS SP 

References and documentation are available.

Number of amino acids: 332

Molecular weight: 34777.46

Theoretical pI: 5.86

Amino acid composition: 
Ala (A)  28	  8.4%
Arg (R)  20	  6.0%
Asn (N)   9	  2.7%
Asp (D)  21	  6.3%
Cys (C)   7	  2.1%
Gln (Q)   7	  2.1%
Glu (E)  12	  3.6%
Gly (G)  38	 11.4%
His (H)   4	  1.2%
Ile (I)   4	  1.2%
Leu (L)  23	  6.9%
Lys (K)  10	  3.0%
Met (M)  13	  3.9%
Phe (F)   6	  1.8%
Pro (P)  41	 12.3%
Ser (S)  51	 15.4%
Thr (T)  13	  3.9%
Trp (W)   1	  0.3%
Tyr (Y)  17	  5.1%
Val (V)   7	  2.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1490
Hydrogen    H	      2305
Nitrogen    N	       427
Oxygen      O	       496
Sulfur      S	        20

Formula: C1490H2305N427O496S20
Total number of atoms: 4738

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31205
Abs 0.1% (=1 g/l)   0.897, assuming all pairs of Cys residues form cystines


Ext. coefficient    30830
Abs 0.1% (=1 g/l)   0.886, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is D (Asp).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 78.30
This classifies the protein as unstable.



Aliphatic index: 46.27

Grand average of hydropathicity (GRAVY): -0.671