ProtParam
User-provided sequence:
10 20 30 40 50 60
EEMEEAANQK LALQKAKEVA EVSPMSAANI SIAAFVKQTR GTVSRSSSVS SVNSPRQQNS
References and
documentation are available.
Number of amino acids: 60
Molecular weight: 6379.10
Theoretical pI: 8.53
Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 7
Atomic composition:
Carbon C 266
Hydrogen H 448
Nitrogen N 82
Oxygen O 95
Sulfur S 2
Formula: C
266H
448N
82O
95S
2
Total number of atoms: 893
Extinction coefficients:
As there are no Trp, Tyr or Cys in the region considered, your protein
should not be visible by UV spectrophotometry.
Estimated half-life:
The N-terminal of the sequence considered is E (Glu).
The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 77.46
This classifies the protein as unstable.
Aliphatic index: 70.00
Grand average of hydropathicity (GRAVY): -0.513