ProtParam

User-provided sequence:

        10         20         30         40         50         60 
EGARVTSPTS GLRRLSLMDQ VSGVEAFLQV SQPPDPHRGA KVIRADPQGG RRRHSSETFS 

        70         80         90        100        110        120 
STTTVTPVSP SFAHNSKRHN SASVENVSLR KSSEGNSILG GSDEPSTSPG QAQPSAGVPP 

       130        140        150 
GPQARPWNPG QPGALIGCPG GSSSPMRRET SVGF 

References and documentation are available.

Number of amino acids: 154

Molecular weight: 15963.57

Theoretical pI: 10.66

Amino acid composition: 
Ala (A)  10	  6.5%
Arg (R)  13	  8.4%
Asn (N)   5	  3.2%
Asp (D)   4	  2.6%
Cys (C)   1	  0.6%
Gln (Q)   8	  5.2%
Glu (E)   7	  4.5%
Gly (G)  18	 11.7%
His (H)   4	  2.6%
Ile (I)   3	  1.9%
Leu (L)   7	  4.5%
Lys (K)   3	  1.9%
Met (M)   2	  1.3%
Phe (F)   4	  2.6%
Pro (P)  18	 11.7%
Ser (S)  26	 16.9%
Thr (T)   9	  5.8%
Trp (W)   1	  0.6%
Tyr (Y)   0	  0.0%
Val (V)  11	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       676
Hydrogen    H	      1086
Nitrogen    N	       218
Oxygen      O	       225
Sulfur      S	         3

Formula: C676H1086N218O225S3
Total number of atoms: 2208

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     5500
Abs 0.1% (=1 g/l)   0.345, assuming all pairs of Cys residues form cystines


Ext. coefficient     5500
Abs 0.1% (=1 g/l)   0.345, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is E (Glu).

The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 73.67
This classifies the protein as unstable.



Aliphatic index: 52.53

Grand average of hydropathicity (GRAVY): -0.709