ProtParam

User-provided sequence:

        10         20         30         40         50         60 
FGSREPPQPK PSLSSHPISP TAALEPGPHP QGSVWTVRPL PTTCLRIHSP LKRPWPPTLA 

        70         80         90        100        110        120 
APPGLPMCPP SLPNPTLSAV YPMATTALWD SAPASGRGTA TTTQTRITGA SCTTTTHAPA 

       130        140        150        160        170 
RGCGFQLMSF IIRSGLCGHS PGRRVLTSRP LWWRFLLLSL PPFYTGQTNK ALS 

References and documentation are available.

Number of amino acids: 173

Molecular weight: 18463.38

Theoretical pI: 11.12

Amino acid composition: 
Ala (A)  14	  8.1%
Arg (R)  12	  6.9%
Asn (N)   2	  1.2%
Asp (D)   1	  0.6%
Cys (C)   5	  2.9%
Gln (Q)   5	  2.9%
Glu (E)   2	  1.2%
Gly (G)  13	  7.5%
His (H)   5	  2.9%
Ile (I)   5	  2.9%
Leu (L)  19	 11.0%
Lys (K)   3	  1.7%
Met (M)   3	  1.7%
Phe (F)   5	  2.9%
Pro (P)  29	 16.8%
Ser (S)  18	 10.4%
Thr (T)  21	 12.1%
Trp (W)   5	  2.9%
Tyr (Y)   2	  1.2%
Val (V)   4	  2.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 3
Total number of positively charged residues (Arg + Lys): 15

Atomic composition:

Carbon      C	       830
Hydrogen    H	      1302
Nitrogen    N	       234
Oxygen      O	       228
Sulfur      S	         8

Formula: C830H1302N234O228S8
Total number of atoms: 2602

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30730
Abs 0.1% (=1 g/l)   1.664, assuming all pairs of Cys residues form cystines


Ext. coefficient    30480
Abs 0.1% (=1 g/l)   1.651, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is F (Phe).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.85
This classifies the protein as unstable.



Aliphatic index: 68.90

Grand average of hydropathicity (GRAVY): -0.206