ProtParam

User-provided sequence:

        10         20         30         40         50         60 
FLCHVAIAGR TCPKPDELPF AVVVPLKTFY DPGEQIVYSC KPGYVSRGGM RRFTCPLTGM 

        70         80         90        100        110        120 
WPINTLKCIP RVCPFAGILE NGVVRYTTFE YPNTIGFACN PGYYLNGTSS SKCTEEGKWS 

       130        140        150        160        170        180 
PELPVCARIT CPPPPIPKFA ALKEYKTSVG NSSFYQDTVV FKCLPHFAMF GNDTVTCTAH 

       190        200        210        220        230        240 
GNWTQLPECR EVKCPFPSRP DNGFVNYPAK PVLSYKDKAV FGCHETYKLD GPEEVECTKT 

       250        260 
GNWSALPSCK ASCKLSVKKA TVLYQG 

References and documentation are available.

Number of amino acids: 266

Molecular weight: 29393.99

Theoretical pI: 8.66

Amino acid composition: 
Ala (A)  15	  5.6%
Arg (R)   9	  3.4%
Asn (N)  11	  4.1%
Asp (D)   7	  2.6%
Cys (C)  18	  6.8%
Gln (Q)   4	  1.5%
Glu (E)  14	  5.3%
Gly (G)  20	  7.5%
His (H)   4	  1.5%
Ile (I)   8	  3.0%
Leu (L)  16	  6.0%
Lys (K)  20	  7.5%
Met (M)   3	  1.1%
Phe (F)  15	  5.6%
Pro (P)  28	 10.5%
Ser (S)  15	  5.6%
Thr (T)  21	  7.9%
Trp (W)   4	  1.5%
Tyr (Y)  13	  4.9%
Val (V)  21	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1328
Hydrogen    H	      2024
Nitrogen    N	       340
Oxygen      O	       373
Sulfur      S	        21

Formula: C1328H2024N340O373S21
Total number of atoms: 4086

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42495
Abs 0.1% (=1 g/l)   1.446, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   1.407, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is F (Phe).

The estimated half-life is: 1.1 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.53
This classifies the protein as unstable.



Aliphatic index: 63.72

Grand average of hydropathicity (GRAVY): -0.198