ProtParam

User-provided sequence:

        10         20         30         40         50         60 
GEEGASGAGL GPSKRVLGSL LSDSLFLEVK ELFLAAVRLD PTSIDPDVQC GLGVLFNLSG 

        70         80         90        100        110        120 
EYDKAVDCFT AALSVRPNDY LLWNKLGATL ANGNQSEEAV AAYRRALELQ PGYIRSRYNL 

       130        140        150        160        170        180 
GISCINLGAH REAVEHFLEA LNMQRKSRGP RGEGGAMSEN IWSTLRLALS MLGQSDAYGA 

       190 
ADARDLPALL TMFGLPQ 

References and documentation are available.

Number of amino acids: 197

Molecular weight: 21131.98

Theoretical pI: 5.10

Amino acid composition: 
Ala (A)  23	 11.7%
Arg (R)  13	  6.6%
Asn (N)   9	  4.6%
Asp (D)  10	  5.1%
Cys (C)   3	  1.5%
Gln (Q)   6	  3.0%
Glu (E)  13	  6.6%
Gly (G)  21	 10.7%
His (H)   2	  1.0%
Ile (I)   5	  2.5%
Leu (L)  31	 15.7%
Lys (K)   5	  2.5%
Met (M)   4	  2.0%
Phe (F)   6	  3.0%
Pro (P)   8	  4.1%
Ser (S)  16	  8.1%
Thr (T)   5	  2.5%
Trp (W)   2	  1.0%
Tyr (Y)   6	  3.0%
Val (V)   9	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       930
Hydrogen    H	      1480
Nitrogen    N	       262
Oxygen      O	       286
Sulfur      S	         7

Formula: C930H1480N262O286S7
Total number of atoms: 2965

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    20065
Abs 0.1% (=1 g/l)   0.950, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.944, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.07
This classifies the protein as stable.



Aliphatic index: 96.19

Grand average of hydropathicity (GRAVY): -0.066