ProtParam

User-provided sequence:

        10         20         30         40         50 
GRIVAFFEFG GVMCVESVNR EMSPLVDNIA LWMTEYLNRH LHTWIQDNGG WVGACLVE 

References and documentation are available.

Number of amino acids: 58

Molecular weight: 6612.60

Theoretical pI: 4.74

Amino acid composition: 
Ala (A)   3	  5.2%
Arg (R)   3	  5.2%
Asn (N)   4	  6.9%
Asp (D)   2	  3.4%
Cys (C)   2	  3.4%
Gln (Q)   1	  1.7%
Glu (E)   5	  8.6%
Gly (G)   6	 10.3%
His (H)   2	  3.4%
Ile (I)   3	  5.2%
Leu (L)   5	  8.6%
Lys (K)   0	  0.0%
Met (M)   3	  5.2%
Phe (F)   3	  5.2%
Pro (P)   1	  1.7%
Ser (S)   2	  3.4%
Thr (T)   2	  3.4%
Trp (W)   3	  5.2%
Tyr (Y)   1	  1.7%
Val (V)   7	 12.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 7
Total number of positively charged residues (Arg + Lys): 3

Atomic composition:

Carbon      C	       297
Hydrogen    H	       447
Nitrogen    N	        79
Oxygen      O	        83
Sulfur      S	         5

Formula: C297H447N79O83S5
Total number of atoms: 911

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    18115
Abs 0.1% (=1 g/l)   2.739, assuming all pairs of Cys residues form cystines


Ext. coefficient    17990
Abs 0.1% (=1 g/l)   2.721, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.56
This classifies the protein as unstable.



Aliphatic index: 93.97

Grand average of hydropathicity (GRAVY): 0.233