ProtParam
User-provided sequence:
10 20 30 40 50 60
GSSGLAXARL LSRTFLLQQN GIRHGSYTAS RKNIYIXKNT KVICQGFTGK QGTFHSQQAL
EYGTK
References and
documentation are available.
Number of amino acids: 65
Molecular weight: 7155.54
Theoretical pI: 10.46
Total number of negatively charged residues (Asp + Glu): 1
Total number of positively charged residues (Arg + Lys): 9
Atom composition:
As there is at least one ambiguous position (B,Z or X) in the sequence
considered, the atomic composition cannot be computed.
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.625, assuming all pairs of Cys residues form cystines
Ext. coefficient 4470
Abs 0.1% (=1 g/l) 0.625, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is G (Gly).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.36
This classifies the protein as stable.
Aliphatic index: 70.62
Grand average of hydropathicity (GRAVY): -0.491