ProtParam

User-provided sequence:

        10         20         30         40         50         60 
IRTKPEASGT DNEDKVMEIK GQMIHVPESN AILFLGSPCV DKLDELIGRG LHLSDIPIHD 

        70         80         90        100        110        120 
ATRDVILVGE QAKAQDGLKK RMDKLKATLE KTHQALEEEK KKTVDLLYSI FPGDVAQQLW 

       130        140        150        160        170        180 
QRQQVQARKF DDVTMLFSDI VGFTAICAQC TPMQVISMLN ELYTRFDHQC GFLDIYKMRI 

       190        200        210        220 
GIHSGSVLAG VVGVRMPRYC LFGNNVTLAS KFESGSHPRL 

References and documentation are available.

Number of amino acids: 220

Molecular weight: 24646.52

Theoretical pI: 6.72

Amino acid composition: 
Ala (A)  13	  5.9%
Arg (R)  11	  5.0%
Asn (N)   5	  2.3%
Asp (D)  16	  7.3%
Cys (C)   5	  2.3%
Gln (Q)  13	  5.9%
Glu (E)  12	  5.5%
Gly (G)  16	  7.3%
His (H)   7	  3.2%
Ile (I)  15	  6.8%
Leu (L)  22	 10.0%
Lys (K)  16	  7.3%
Met (M)   8	  3.6%
Phe (F)   9	  4.1%
Pro (P)   8	  3.6%
Ser (S)  12	  5.5%
Thr (T)  11	  5.0%
Trp (W)   1	  0.5%
Tyr (Y)   4	  1.8%
Val (V)  16	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1089
Hydrogen    H	      1754
Nitrogen    N	       302
Oxygen      O	       322
Sulfur      S	        13

Formula: C1089H1754N302O322S13
Total number of atoms: 3480

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11710
Abs 0.1% (=1 g/l)   0.475, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.465, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is I (Ile).

The estimated half-life is: 20 hours (mammalian reticulocytes, in vitro).
                            30 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.54
This classifies the protein as stable.



Aliphatic index: 92.59

Grand average of hydropathicity (GRAVY): -0.197