ProtParam

User-provided sequence:

        10         20         30         40         50         60 
LLEVIDTRTQ ILAASYELHK FYHDAKEIFG RIQDKHKKLP EELGRDQNTV ETLQRMHTTF 

        70         80         90        100        110        120 
EHDIQALGTQ VRQLQEDAAR LQAAYAGDKA DDIQKRENEV LEAWKSLLDA CEGRRVRLVD 

       130        140        150        160        170        180 
TGDKFRFFSM VRDLMLWMED VIRQIEAQEK PRDVSSVELL MNNHQGIKAE IDARNDSFTA 

       190        200 
CIELGKALLA RKHYASEEIK 

References and documentation are available.

Number of amino acids: 200

Molecular weight: 23210.31

Theoretical pI: 5.56

Amino acid composition: 
Ala (A)  19	  9.5%
Arg (R)  16	  8.0%
Asn (N)   5	  2.5%
Asp (D)  17	  8.5%
Cys (C)   2	  1.0%
Gln (Q)  13	  6.5%
Glu (E)  20	 10.0%
Gly (G)   8	  4.0%
His (H)   7	  3.5%
Ile (I)  12	  6.0%
Leu (L)  21	 10.5%
Lys (K)  14	  7.0%
Met (M)   5	  2.5%
Phe (F)   7	  3.5%
Pro (P)   2	  1.0%
Ser (S)   7	  3.5%
Thr (T)   9	  4.5%
Trp (W)   2	  1.0%
Tyr (Y)   4	  2.0%
Val (V)  10	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1015
Hydrogen    H	      1628
Nitrogen    N	       296
Oxygen      O	       313
Sulfur      S	         7

Formula: C1015H1628N296O313S7
Total number of atoms: 3259

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17085
Abs 0.1% (=1 g/l)   0.736, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.731, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is L (Leu).

The estimated half-life is: 5.5 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.98
This classifies the protein as unstable.



Aliphatic index: 88.35

Grand average of hydropathicity (GRAVY): -0.613